Human Protein Kinase B Beta (PKBb) ELISA Kit | PKBb elisa kit

Human Protein Kinase B Beta (PKBb) ELISA Kit

Cat. Num. AAA2019024

• Reactivity: Human
• Synonyms: Protein Kinase B Beta (PKBb); Human Protein Kinase B Beta (PKBb) ELISA Kit; AKT2; PKBBETA; PRKB-B; RAC-BETA; RAC-Beta Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 2; PKBb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Beta (PKBb).; No significant cross-reactivity or interference between Protein Kinase B Beta (PKBb) and analogues was observed.
• Sequence Length: 481

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKBb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Beta (PKBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Beta (PKBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Beta (PKBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Beta (PKBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PKBb elisa kit

Enzyme & Kinase; Metabolic pathway; Endocrinology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 76365214
• NCBI GeneID: 25233
• UniProt Accession #: P47197
• Molecular Weight: 55,543 Da
• NCBI Official Full Name: protein kinase B beta
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 2
• NCBI Official Symbol: Akt2
• NCBI Protein Information: RAC-beta serine/threonine-protein kinase
• UniProt Protein Name: RAC-beta serine/threonine-protein kinase
• UniProt Gene Name: Akt2
• UniProt Synonym Gene Names: PKB beta
• UniProt Entry Name: AKT2_RAT

NCBI Description

may be involved in phosphatidylinositol 3-kinase (PI3-K) mediated signaling [RGD, Feb 2006]

Uniprot Description

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; plasma membrane; cell cortex; nucleus; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C binding; ATP binding; protein kinase activity

Biological Process: response to muscle activity; positive regulation of positive chemotaxis; positive regulation of nitric oxide biosynthetic process; multicellular organismal development; positive regulation of glycogen biosynthetic process; intracellular protein transport across a membrane; positive regulation of vesicle fusion; negative regulation of caspase activity; protein amino acid phosphorylation; response to insulin stimulus; regulation of translation; positive regulation of glucose import; negative regulation of RNA splicing; protein kinase B signaling cascade; response to nutrient levels; glycogen biosynthetic process; positive regulation of signal transduction; carbohydrate transport; myelin maintenance in the peripheral nervous system; glucose metabolic process; response to osmotic stress; positive regulation of peptidyl-serine phosphorylation; positive regulation of fatty acid beta-oxidation; cellular response to hormone stimulus; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of cell migration; negative regulation of apoptosis