NR2C1 blocking peptide

NR2C1 Blocking Peptide

Cat. Num. AAA9627811

• Gene Names: NR2C1; TR2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: NR2C1; NR2C1 Blocking Peptide; NR2C 1; NR2C1_HUMAN; NUCLEAR HORMONE RECEPTOR TR2; Nuclear receptor subfamily 2 Group C isoform; Nuclear receptor subfamily 2 group C member 1; Orphan nuclear receptor TR2; Testicular receptor 2; TR 2; TR2; NR2C1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for NR2C1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NR2C1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 73808084
• NCBI GeneID: 7181
• NCBI Accession #: NP_001027458.1
• NCBI GenBank Nucleotide #: NM_001032287.2
• UniProt Accession #: P13056; Q15625; Q15626; A8K5K4
• Molecular Weight: 67,315 Da
• NCBI Official Full Name: nuclear receptor subfamily 2 group C member 1 isoform b
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2, group C, member 1
• NCBI Official Symbol: NR2C1
• NCBI Official Synonym Symbols: TR2
• NCBI Protein Information: nuclear receptor subfamily 2 group C member 1; orphan nuclear receptor TR2; TR2 nuclear hormone receptor; nuclear receptor subfamily 2, group C isoform
• UniProt Protein Name: Nuclear receptor subfamily 2 group C member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR2C1
• UniProt Synonym Gene Names: TR2
• UniProt Entry Name: NR2C1_HUMAN

Uniprot Description

NR2C1: Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid- regulated preadipocyte proliferation. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; PML body

Molecular Function: protein binding; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; steroid hormone receptor activity; receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; positive regulation of retinoic acid receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter