NCF4 blocking peptide

NCF4 Antibody (C-term) Blocking Peptide

• Gene Names: NCF4; NCF; CGD3; P40PHOX; SH3PXD4
• Synonyms: NCF4; NCF4 Antibody (C-term) Blocking Peptide; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox; SH3PXD4; NCF4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human NCF4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 339

• Cellular Location: Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane; Peripheral membrane protein
• Tissue Location: Expression is restricted to hematopoietic cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NCF4 blocking peptide

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

NCBI and Uniprot Product Information

• NCBI GI #: 108884815
• NCBI GeneID: 4689
• UniProt Accession #: Q15080; O60808; Q86U56; Q9BU98; Q9NP45; A8K4F9
• Molecular Weight: 39,017 Da
• NCBI Official Full Name: Neutrophil cytosol factor 4
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 4
• NCBI Official Symbol: NCF4
• NCBI Official Synonym Symbols: NCF; CGD3; P40PHOX; SH3PXD4
• NCBI Protein Information: neutrophil cytosol factor 4
• UniProt Protein Name: Neutrophil cytosol factor 4
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF4
• UniProt Synonym Gene Names: SH3PXD4; NCF-4; p40phox
• UniProt Entry Name: NCF4_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytosol; endosome membrane; membrane; NADPH oxidase complex

Molecular Function: phosphatidylinositol 3-phosphate binding; protein binding; protein dimerization activity; superoxide-generating NADPH oxidase activator activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; immune response; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii