Rabbit NCF4 Polyclonal Antibody | anti-NCF4 antibody

NCF4 Rabbit Polyclonal

Cat. Num. AAA7601815

• Gene Names: NCF4; NCF; CGD3; P40PHOX; SH3PXD4
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: NCF4; Polyclonal Antibody; NCF4 Rabbit Polyclonal; anti-NCF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 348

• Applicable Applications for anti-NCF4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Neutrophil cytosolic factor 4, 40kDa
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart using MBS7601815 (NCF4 antibody) at dilution of 1:50)

Western Blot (WB)

(L02 cells were subjected to SDS PAGE followed by western blot with MBS7601815 (NCF4 Antibody) at dilution of 1:300)

NCBI and Uniprot Product Information

• NCBI GI #: 163644267
• NCBI GeneID: 4689
• NCBI Accession #: NP_038202.2
• NCBI GenBank Nucleotide #: NM_013416.3
• Molecular Weight: 39,017 Da
• NCBI Official Full Name: neutrophil cytosol factor 4 isoform 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 4
• NCBI Official Symbol: NCF4
• NCBI Official Synonym Symbols: NCF; CGD3; P40PHOX; SH3PXD4
• NCBI Protein Information: neutrophil cytosol factor 4
• UniProt Protein Name: Neutrophil cytosol factor 4
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF4
• UniProt Synonym Gene Names: SH3PXD4; NCF-4; p40phox
• UniProt Entry Name: NCF4_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytosol; endosome membrane; membrane; NADPH oxidase complex

Molecular Function: phosphatidylinositol 3-phosphate binding; protein binding; protein dimerization activity; superoxide-generating NADPH oxidase activator activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; immune response; positive regulation of catalytic activity; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii