MAGEL2 blocking peptide

MAGEL2 Antibody (C-term) Blocking Peptide

• Gene Names: MAGEL2; PWLS; nM15; NDNL1; SHFYNG
• Synonyms: MAGEL2; MAGEL2 Antibody (C-term) Blocking Peptide; MAGE-like protein 2; Necdin-like protein 1; Protein nM15; NDNL1; MAGEL2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human MAGEL2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 1249

• Cellular Location: Early endosome. Cytoplasm. Nucleus. Note: Recruited to retromer- containing endosomes via interaction with VPS35. Colocalizes with CLOCK and ARNTL/BMLA1 in the cytoplasm, and with PER2 in the cytoplasm and nucleus (By similarity)
• Tissue Location: Expressed in placenta, fetal and adult brain. Not detected in heart and small intestine, very low levels in fibroblasts. Not expressed in brain of a Prader-Willi patient
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAGEL2 blocking peptide

Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys- 63'-linked polyubiquitin chains at 'Lys-220' of WASH1 together with TRIM27, leading to promote endosomal F-actin assembly (PubMed:23452853). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 928505947
• NCBI GeneID: 54551
• UniProt Accession #: Q9UJ55; H0YDD5
• Molecular Weight: 132,822 Da
• NCBI Official Full Name: MAGE-like protein 2
• NCBI Official Synonym Full Names: MAGE family member L2
• NCBI Official Symbol: MAGEL2
• NCBI Official Synonym Symbols: PWLS; nM15; NDNL1; SHFYNG
• NCBI Protein Information: MAGE-like protein 2
• UniProt Protein Name: MAGE-like protein 2
• Protein Family: MAGE-like protein
• UniProt Gene Name: MAGEL2
• UniProt Synonym Gene Names: NDNL1
• UniProt Entry Name: MAGL2_HUMAN

NCBI Description

Prader-Willi syndrome (PWS) is caused by the loss of expression of imprinted genes in chromosome 15q11-q13 region. Affected individuals exhibit neonatal hypotonia, developmental delay, and childhood-onset obesity. Necdin (NDN), a gene involved in the terminal differentiation of neurons, localizes to this region of the genome and has been implicated as one of the genes responsible for the etiology of PWS. This gene is structurally similar to NDN, is also localized to the PWS chromosomal region, and is paternally imprinted, suggesting a possible role for it in PWS. [provided by RefSeq, Oct 2010]

Uniprot Description

MAGE-L2: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Interacts with TRIM27

Chromosomal Location of Human Ortholog: 15q11.2

Cellular Component: endosome; nucleus; retromer complex

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; retrograde transport, endosome to Golgi

Disease: Prader-willi Syndrome; Prader-willi-like Syndrome