ITGB1BP1 blocking peptide

ITGB1BP1 Antibody (Center) Blocking Peptide

• Gene Names: ITGB1BP1; ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• Synonyms: ITGB1BP1; ITGB1BP1 Antibody (Center) Blocking Peptide; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1; ICAP-1; ICAP1; ITGB1BP1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 200

• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Note: Nucleocytoplasmic shuttling protein; shuttles between nucleus and cytoplasm in a integrin-dependent manner; probably sequestered in the cytosol by ITGB1. Its localization is dependent on the stage of cell spreading on fibronectin; cytoplasmic in case of round cells, corresponding to the initial step of cell spreading, or nuclear in case of well spread cells. Colocalizes with ROCK1 and NME2 at beta-1 integrin engagement sites. Together with ITGB1 and NME2 is recruited to beta-1 integrin-rich peripheral ruffles and lamellipodia during initial cell spreading on fibronectin and/or collagen
• Tissue Location: Expressed in endothelial cells and fibroblasts (at protein level). Ubiquitously expressed. Expressed in intestine, colon, testis, ovary, thymus, spleen and prostate
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ITGB1BP1 blocking peptide

Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin- dependent manner. Involved in the regulation of beta-1 integrin- containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 17366148
• NCBI GeneID: 9270
• UniProt Accession #: O14713; O14714; Q53RS0; D6W4Y9
• Molecular Weight: 16,140 Da
• NCBI Official Full Name: Integrin beta-1-binding protein 1
• NCBI Official Synonym Full Names: integrin subunit beta 1 binding protein 1
• NCBI Official Symbol: ITGB1BP1
• NCBI Official Synonym Symbols: ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• NCBI Protein Information: integrin beta-1-binding protein 1
• UniProt Protein Name: Integrin beta-1-binding protein 1
• Protein Family: Integrin beta-1-binding protein
• UniProt Gene Name: ITGB1BP1
• UniProt Synonym Gene Names: ICAP1; ICAP-1
• UniProt Entry Name: ITBP1_HUMAN

NCBI Description

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion. Several transcript variants, some protein-coding and some non-protein coding, have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

ICAP1: May play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: cytoplasm; cytoskeleton; cytosol; focal adhesion; lamellipodium; nucleus; perinuclear region of cytoplasm; plasma membrane; ruffle

Molecular Function: GDP-dissociation inhibitor activity; integrin binding; protein binding; protein complex binding; protein transporter activity

Biological Process: activation of protein kinase B; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell migration; cell-matrix adhesion; integrin activation; integrin-mediated signaling pathway; lumen formation; myoblast migration; negative regulation of cell proliferation; negative regulation of focal adhesion formation; negative regulation of protein binding; negative regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of Notch signaling pathway; positive regulation of protein kinase B signaling cascade; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; protein transport; receptor clustering; regulation of blood vessel size; regulation of cell adhesion mediated by integrin; regulation of GTPase activity; substrate-bound cell migration, cell release from substrate