ICMT blocking peptide

ICMT (C-term) Antibody Blocking Peptide

• Gene Names: ICMT; PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098
• Synonyms: ICMT; ICMT (C-term) Antibody Blocking Peptide; Protein-S-isoprenylcysteine O-methyltransferase; Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT; PCCMT; ICMT blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human ICMT (C-term). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 284

• Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Tissue Location: Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions Abundant expression seen in the Purkinje cells and pontine neurons.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ICMT blocking peptide

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

NCBI and Uniprot Product Information

• NCBI GI #: 14548077
• NCBI GeneID: 23463
• UniProt Accession #: O60725; Q6FHT0
• Molecular Weight: 31,938 Da
• NCBI Official Full Name: Protein-S-isoprenylcysteine O-methyltransferase
• NCBI Official Synonym Full Names: isoprenylcysteine carboxyl methyltransferase
• NCBI Official Symbol: ICMT
• NCBI Official Synonym Symbols: PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098
• NCBI Protein Information: protein-S-isoprenylcysteine O-methyltransferase
• UniProt Protein Name: Protein-S-isoprenylcysteine O-methyltransferase
• Protein Family: Protein-S-isoprenylcysteine O-methyltransferase
• UniProt Gene Name: ICMT
• UniProt Synonym Gene Names: PCCMT; PPMT; pcCMT
• UniProt Entry Name: ICMT_HUMAN

NCBI Description

This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ICMT: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Belongs to the isoprenylcysteine O-methyltransferase family.

Protein type: Membrane protein, multi-pass; EC 2.1.1.100; Methyltransferase; Endoplasmic reticulum; Transcription, coactivator/corepressor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane

Molecular Function: C-terminal protein carboxyl methyltransferase activity; protein-S-isoprenylcysteine O-methyltransferase activity

Biological Process: post-translational protein modification; protein modification process; protein targeting to membrane