HIRA blocking peptide

HIRA Antibody (N-term) Blocking Peptide

• Gene Names: HIRA; TUP1; DGCR1; TUPLE1
• Synonyms: HIRA; HIRA Antibody (N-term) Blocking Peptide; Protein HIRA; TUP1-like enhancer of split protein 1; DGCR1; HIR; TUPLE1; HIRA blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1017

• Cellular Location: Nucleus. Nucleus, PML body. Note: Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence
• Tissue Location: Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HIRA blocking peptide

Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

NCBI and Uniprot Product Information

• NCBI GI #: 88984228
• NCBI GeneID: 7290
• UniProt Accession #: P54198; Q05BU9; Q8IXN2
• Molecular Weight: 89,299 Da
• NCBI Official Full Name: Protein HIRA
• NCBI Official Synonym Full Names: histone cell cycle regulator
• NCBI Official Symbol: HIRA
• NCBI Official Synonym Symbols: TUP1; DGCR1; TUPLE1
• NCBI Protein Information: protein HIRA
• UniProt Protein Name: Protein HIRA
• Protein Family: HIRA-interacting protein
• UniProt Gene Name: HIRA
• UniProt Synonym Gene Names: DGCR1; HIR; TUPLE1
• UniProt Entry Name: HIRA_HUMAN

NCBI Description

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.

Protein type: Transcription regulation; Cell cycle regulation; Cell development/differentiation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function: protein binding; transcription corepressor activity; transcription factor activity

Biological Process: anatomical structure morphogenesis; DNA replication-independent nucleosome assembly; regulation of transcription from RNA polymerase II promoter