GTF2I blocking peptide

GTF2I Antibody (C-term) Blocking Peptide

• Gene Names: GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
• Synonyms: GTF2I; GTF2I Antibody (C-term) Blocking Peptide; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein; BAP135; WBSCR6; GTF2I blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human GTF2I. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 998

• Cellular Location: Cytoplasm. Nucleus. Note: Colocalizes with BTK in the cytoplasm
• Tissue Location: Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GTF2I blocking peptide

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

NCBI and Uniprot Product Information

• NCBI GI #: 17865459
• NCBI GeneID: 2969
• UniProt Accession #: P78347; O14743; O15359; O43546; O43588; O43589; Q75M85; Q75M86; Q75M87; Q75M88; Q86U51; Q9BSZ4
• Molecular Weight: 30,409 Da
• NCBI Official Full Name: General transcription factor II-I
• NCBI Official Synonym Full Names: general transcription factor IIi
• NCBI Official Symbol: GTF2I
• NCBI Official Synonym Symbols: WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
• NCBI Protein Information: general transcription factor II-I
• UniProt Protein Name: General transcription factor II-I
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2I
• UniProt Synonym Gene Names: BAP135; WBSCR6; GTFII-I; TFII-I; BAP-135; BTK-associated protein 135; SPIN
• UniProt Entry Name: GTF2I_HUMAN

NCBI Description

This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]

Uniprot Description

GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding

Biological Process: negative regulation of angiogenesis; signal transduction; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

Disease: Williams-beuren Syndrome