FBP2 blocking peptide

FBP2 Antibody (C-term) Blocking Peptide

• Synonyms: FBP2; FBP2 Antibody (C-term) Blocking Peptide; Fructose-1;6-bisphosphatase isozyme 2; FBPase 2; D-fructose-1;6-bisphosphate 1-phosphohydrolase 2; Muscle FBPase; FBP2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human FBP2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 339

• Cellular Location: Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. Note: In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration.
• Tissue Location: Expressed in skeletal muscle (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FBP2 blocking peptide

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.

NCBI and Uniprot Product Information

• NCBI GI #: 76789651
• NCBI GeneID: 8789
• UniProt Accession #: O00757; Q17R39; Q6FI53
• Molecular Weight: 36,743 Da
• NCBI Official Full Name: Fructose-1,6-bisphosphatase isozyme 2
• NCBI Official Synonym Full Names: fructose-bisphosphatase 2
• NCBI Official Symbol: FBP2
• NCBI Protein Information: fructose-1,6-bisphosphatase isozyme 2
• UniProt Protein Name: Fructose-1,6-bisphosphatase isozyme 2
• Protein Family: Fat body protein
• UniProt Gene Name: FBP2
• UniProt Synonym Gene Names: FBPase 2
• UniProt Entry Name: F16P2_HUMAN

NCBI Description

This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]

Uniprot Description

FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.

Protein type: EC 3.1.3.11; Carbohydrate Metabolism - pentose phosphate pathway; Phosphatase (non-protein); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytosol

Molecular Function: fructose-bisphosphatase activity; protein binding

Biological Process: fructose metabolic process; gluconeogenesis