Rabbit anti-Human TIMP1 Polyclonal Antibody | anti-TIMP1 antibody

TIMP1, NT (Metalloproteinase Inhibitor 1, Tissue Inhibitor Of Metalloproteinases 1, TIMP-1, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor, Collagenase Inhibitor, TIMP, CLGI) (PE)

Cat. Num. AAA6502717

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TIMP1; Polyclonal Antibody; NT (Metalloproteinase Inhibitor 1; Tissue Inhibitor Of Metalloproteinases 1; TIMP-1; Erythroid-potentiating Activity; EPA; Fibroblast Collagenase Inhibitor; Collagenase Inhibitor; TIMP; CLGI) (PE); anti-TIMP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TIMP1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TIMP1 antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; IF: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa50-80 from the N-terminal region of human TIMP1 conjugated to KLH. Cellular Localization: secreted
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TIMP1 Antibody (N-term) in CEM cell line lysates (35ug/lane). TIMP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using TIMP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TIMP1 Antibody (N-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-TIMP1 antibody

Antibodies; Matrix Metalloproteinases

References

1. Safranek, J., Anticancer Res. 29(7), 2513-2517 (2009). 2. Onal, I.K., Eur. J. Intern. Med. 20(4), 369-372 (2009). 3. Okamura, H., Int. J. Oncol. 35(1), 181-186 (2009). 4. Wu T et al., J Mol Neurosci 2011 Aug 16.

NCBI and Uniprot Product Information

• NCBI GI #: 4507509
• NCBI GeneID: 7076
• NCBI Accession #: NP_003245
• NCBI GenBank Nucleotide #: NM_003254.2
• UniProt Accession #: P01033; Q14252; Q9UCU1
• Molecular Weight: 23kDa (205aa)
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• NCBI Protein Information: metalloproteinase inhibitor 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; platelet activation; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis