ETFDH blocking peptide

ETFDH Blocking Peptide

Cat. Num. AAA9621100

• Gene Names: ETFDH; MADD; ETFQO
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ETFDH; ETFDH Blocking Peptide; Electron transfer flavoprotein ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase; electron transferring flavoprotein dehydrogenase; Electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase; ETF QO; ETF ubiquinone oxidoreductase; ETF-QO; ETF-ubiquinone oxidoreductase; ETFD_HUMAN; Etfdh; mitochondrial; ETFDH blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ETFDH blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ETFDH blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 528881081
• NCBI GeneID: 2110
• NCBI Accession #: NP_001268666.1
• NCBI GenBank Nucleotide #: NM_001281737.1
• UniProt Accession #: Q16134; Q7Z347; B4E3R9; J3KND9
• Molecular Weight: 62,825 Da
• NCBI Official Full Name: electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: electron transfer flavoprotein dehydrogenase
• NCBI Official Symbol: ETFDH
• NCBI Official Synonym Symbols: MADD; ETFQO
• NCBI Protein Information: electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
• UniProt Protein Name: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
• UniProt Gene Name: ETFDH
• UniProt Synonym Gene Names: ETF-QO; ETF-ubiquinone oxidoreductase
• UniProt Entry Name: ETFD_HUMAN

NCBI Description

This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]

Uniprot Description

ETFDH: Accepts electrons from ETF and reduces ubiquinone. Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C). GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF-QO/FixC family.

Protein type: Mitochondrial; EC 1.5.5.1; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 4q32-q35

Cellular Component: mitochondrial matrix; mitochondrial membrane; integral to mitochondrial inner membrane

Molecular Function: ubiquinone binding; electron carrier activity; FAD binding; metal ion binding; oxidoreductase activity, oxidizing metal ions with flavin as acceptor; 4 iron, 4 sulfur cluster binding; oxidoreductase activity; quinone binding; electron-transferring-flavoprotein dehydrogenase activity

Biological Process: cellular metabolic process; response to oxidative stress; fatty acid beta-oxidation using acyl-CoA dehydrogenase

Disease: Multiple Acyl-coa Dehydrogenase Deficiency