hPD-1 recombinant antibody

Recombinant hPD-1 rabbit Monoclonal Antibody (Clone: ABMRR01)

Cat. Num. AAA668944

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified; Protein A Chromatography
• Synonyms: hPD-1; Monoclonal Recombinant Antibody; Recombinant hPD-1 rabbit Monoclonal Antibody (Clone: ABMRR01); Cancer Marker; PD-1; PDCD1; 5133; Q15116; PD1; CD279; ABMRR01; Recombinant hPD-1 rabbit monoclonal Antibody (Clone: ABMRR01); Low Endotoxin; No Azide; NALE; hPD-1 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: ABMRR01
• Purity/Purification: Purified; Protein A Chromatography
• Form/Format: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 288

• Applicable Applications for hPD-1 recombinant antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 0.5-1 ug/10^6, WB: 1-2 ug/ml
• Antigen: PD-1
• Conjugation: Unconjugated
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Flow Cytometry (FC/FACS)

(Fig-1: Cell surface flow analysis of Rabbit recombinant hPD-1 antibody in PHA treated PBMC (Lymphocyte gated) using 0.5 ug/10^6 cells of Rabbit recombinant hPD-1 antibody (Clone: ABMRR01). Green represent isotype control and red represent Rabbit recombinant hPD-1 antibody. Goat anti rabbit FITC conjugate was used as secondary antibody.)

Flow Cytometry (FC/FACS)

(Fig-2: Cell surface flow analysis of Rabbit recombinant h PD-1 antibody in CHO-PD1 transfected cell line using 0.2 ug/10^6 cells of Rabbit recombinant hPD-1 antibody (Clone: ABMRR01). Green represent CHO/K1 cells and red represent Rabbit recombinant hPD-1 antibody. Goat anti rabbit PE conjugate was used as secondary antibody.)

Western Blot (WB)

(Fig-3: Western blot analysis of Rabbit recombinant hPD-1 antibody. Anti-Rabbit recombinant hPD-1 antibody was tested at 1 ug/ml in Jurkat cell lysate)

Related Product Information for hPD-1 recombinant antibody

PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Product Categories/Family for hPD-1 recombinant antibody

Immune-Check Point

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.