EPHA5 blocking peptide

EphA5 Antibody (N-term) Blocking Peptide

• Gene Names: EPHA5; EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
• Synonyms: EphA5; EphA5 Antibody (N-term) Blocking Peptide; Ephrin type-A receptor 5; Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7; EPHA5; BSK; EHK1; HEK7; TYRO4; EPHA5 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human EphA5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 1037

• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite
• Tissue Location: Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EPHA5 blocking peptide

Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose- stimulated insulin secretion (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 259016353
• NCBI GeneID: 2044
• UniProt Accession #: P54756; Q7Z3F2
• Molecular Weight: 107,699 Da
• NCBI Official Full Name: Ephrin type-A receptor 5
• NCBI Official Synonym Full Names: EPH receptor A5
• NCBI Official Symbol: EPHA5
• NCBI Official Synonym Symbols: EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
• NCBI Protein Information: ephrin type-A receptor 5
• UniProt Protein Name: Ephrin type-A receptor 5
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA5
• UniProt Synonym Gene Names: BSK; EHK1; HEK7; TYRO4; EHK-1; EK7; hEK7
• UniProt Entry Name: EPHA5_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Uniprot Description

EphA5: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 4q13.1

Cellular Component: cell soma; dendrite; external side of plasma membrane; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum

Molecular Function: ephrin receptor activity; GPI-linked ephrin receptor activity

Biological Process: activation of CREB transcription factor; axon guidance; cAMP-mediated signaling; ephrin receptor signaling pathway; hippocampus development; neuron development; regulation of actin cytoskeleton organization and biogenesis; regulation of GTPase activity