Dtnbp1 blocking peptide

Dtnbp1 Antibody (N-term) Blocking Peptide

• Gene Names: Dtnbp1; sdy; Bloc1s8; AW048963; dysbindin; 5430437B18Rik
• Synonyms: Dtnbp1; Dtnbp1 Antibody (N-term) Blocking Peptide; Dysbindin; Biogenesis of lysosome-related organelles complex 1 subunit 8; BLOC-1 subunit 8; Dysbindin-1; Dystrobrevin-binding protein 1; Hermansky-Pudlak syndrome 7 protein homolog; HPS7 protein homolog; Bloc1s8; Sdy; Dtnbp1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human Dtnbp1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 352

• Cellular Location: Isoform 1: Cytoplasm. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Melanosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Endoplasmic reticulum Nucleus. Note: Mainly cytoplasmic but shuttles between the cytoplasm and nucleus. Exported out of the nucleus via its NES in a XPO1-dependent manner. Nuclear localization is required for regulation of the expression of genes such as SYN1. Detected in neuron cell bodies, axons and dendrites. Mainly located to the postsynaptic density. Detected at tubulovesicular elements in the vicinity of the Golgi apparatus and of melanosomes. Occasionally detected at the membrane of pigmented melanosomes in cultured melanoma cells (By similarity). The BLOC-1 complex associates with the BLOC-2 complex in early endosome-associated tubules Associated with the AP-3 complex at presynaptic terminals
• Tissue Location: Detected in brain, in hippocampus and dentate gyrus neurons. Detected at axon bundles and axon terminals, notably in the cerebellum and hippocampus. Detected in neuropil in hippocampus, lateral septum, basal ganglia and substantia nigra Highly expressed in pyramidal cells of hippocampus CA2 and CA3 Detected at the heart and skeletal muscle sarcolemma (at protein level). Ubiquitously expressed. The highest expression is observed in testis, liver, kidney, brain, heart and lung. Expressed at lower levels in stomach and small intestine
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Dtnbp1 blocking peptide

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 38604958
• NCBI GeneID: 94245
• UniProt Accession #: Q91WZ8; Q3TWK1; Q6WXQ1; Q80ZN4; Q9CY43
• Molecular Weight: 30,582 Da
• NCBI Official Full Name: Dysbindin
• NCBI Official Synonym Full Names: dystrobrevin binding protein 1
• NCBI Official Symbol: Dtnbp1
• NCBI Official Synonym Symbols: sdy; Bloc1s8; AW048963; dysbindin; 5430437B18Rik
• NCBI Protein Information: dysbindin
• UniProt Protein Name: Dysbindin
• Protein Family: Dysbindin
• UniProt Gene Name: Dtnbp1
• UniProt Synonym Gene Names: Bloc1s8; Sdy; BLOC-1 subunit 8; HPS7 protein homolog
• UniProt Entry Name: DTBP1_MOUSE

Uniprot Description

DTNBP1: The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. May be required for normal dopamine homeostasis in the cerebral cortex, hippocampus, and hypothalamus. Plays a role in the regulation of cell surface exposure of DRD2. Contributes to the regulation of dopamine signaling. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Part of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). The BLOC-1 complex is composed of BLOC1S1, BLOC1S2, BLOC1S3, DTNBP1, MUTED, PLDN, CNO/cappuccino and SNAPIN. Binds to DTNA and DTNB but may not be a physiological binding partner (PubMed:16980328). Interacts with RNF151 and CMYA5. Identified in a complex with the adapter-related protein complex 3 (AP-3). Interacts with TRIM32, AP3M1 and AP3B2. Identified in a complex with the biogenesis of lysosome-related organelles complex 2 (BLOC-2). Interacts with the DNA-dependent protein kinase complex DNA-PK. Detected in brain, in neurons and in neuropil. Detected in dentate gyrus and in pyramidal cells of hippocampus CA2 and CA3. Belongs to the dysbindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Cellular Component: axon; cytoplasm; dendritic spine; endoplasmic reticulum membrane; growth cone; neuron projection; nucleus; plasma membrane; postsynaptic density; sarcolemma; sarcoplasm; synaptic vesicle membrane

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; anterograde axon cargo transport; anterograde synaptic vesicle transport; blood coagulation; dendrite morphogenesis; muscle development; negative regulation of protein binding; neurite development; neurite morphogenesis; organelle organization and biogenesis; platelet dense granule organization and biogenesis; positive regulation of neurotransmitter secretion; regulation of dopamine receptor signaling pathway; regulation of dopamine secretion; regulation of JNK activity