Dppa3 blocking peptide

Dppa3 Blocking Peptide (C-term)

• Gene Names: Dppa3; PCG7; PGC7; Stella; 2410075G02Rik
• Synonyms: Dppa3; Dppa3 Blocking Peptide (C-term); Developmental pluripotency-associated protein 3; Compaction-associated protein 1; Primordial germ cell protein 7; Cap1p; Crg1; Pgc7; Dppa3 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 134-150 of HUMAN Dppa3
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 150

• Cellular Location: Nucleus. Cytoplasm. Note: Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono- laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In E3.5 blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker
• Tissue Location: Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Dppa3 blocking peptide

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 81866904
• NCBI GeneID: 73708
• UniProt Accession #: Q8QZY3; F6S6F5
• Molecular Weight: 17,670 Da
• NCBI Official Full Name: Developmental pluripotency-associated protein 3
• NCBI Official Synonym Full Names: developmental pluripotency-associated 3
• NCBI Official Symbol: Dppa3
• NCBI Official Synonym Symbols: PCG7; PGC7; Stella; 2410075G02Rik
• NCBI Protein Information: developmental pluripotency-associated protein 3
• UniProt Protein Name: Developmental pluripotency-associated protein 3
• Protein Family: Developmental pluripotency-associated protein
• UniProt Gene Name: Dppa3
• UniProt Synonym Gene Names: Cap1p; Crg1; Pgc7
• UniProt Entry Name: DPPA3_MOUSE

Uniprot Description

Dppa-3:

Cellular Component: cytoplasm; female pronucleus; male pronucleus; nucleus

Molecular Function: methylated histone residue binding; protein binding

Biological Process: embryonic cleavage