Western Blot (WB)
(Western blot analysis of lysates from mouse liver, mouse testis tissue (from left to right), using (Mouse) Dppa3 Antibody (C-term). MBS9205968 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Mouse Dppa3 Polyclonal Antibody | anti-Dppa3 antibody
(Mouse) Dppa3 Antibody (C-term)
Gene Names
Dppa3; PCG7; PGC7; Stella; 2410075G02Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
Dppa3; Polyclonal Antibody; (Mouse) Dppa3 Antibody (C-term); Developmental pluripotency-associated protein 3; Compaction-associated protein 1; Primordial germ cell protein 7; Cap1p; Crg1; Pgc7; anti-Dppa3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This mouse Dppa3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-170 amino acids from the C-terminal region of mouse Dppa3.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
150
Applicable Applications for anti-Dppa3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
Nucleus. Cytoplasm. Note=Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono- laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.
Tissue Location
Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from mouse liver, mouse testis tissue (from left to right), using (Mouse) Dppa3 Antibody (C-term). MBS9205968 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from mouse liver, mouse testis tissue (from left to right), using (Mouse) Dppa3 Antibody (C-term). MBS9205968 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-Dppa3 antibody
Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.
Product Categories/Family for anti-Dppa3 antibody
References
Saitou M.,et al.Nature 418:293-300(2002).
Sato M.,et al.Mech. Dev. 113:91-94(2002).
Bortvin A.,et al.Development 130:1673-1680(2003).
Li W.,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Sato M.,et al.Mech. Dev. 113:91-94(2002).
Bortvin A.,et al.Development 130:1673-1680(2003).
Li W.,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17670
NCBI Official Full Name
developmental pluripotency-associated protein 3
NCBI Official Synonym Full Names
developmental pluripotency-associated 3
NCBI Official Symbol
Dppa3
NCBI Official Synonym Symbols
PCG7; PGC7; Stella; 2410075G02Rik
NCBI Protein Information
developmental pluripotency-associated protein 3
UniProt Protein Name
Developmental pluripotency-associated protein 3
Protein Family
UniProt Gene Name
Dppa3
UniProt Synonym Gene Names
Cap1p; Crg1; Pgc7
UniProt Entry Name
DPPA3_MOUSE
Uniprot Description
Dppa-3:
Cellular Component: male pronucleus; cytoplasm; female pronucleus; nucleus
Molecular Function: protein binding; methylated histone residue binding
Biological Process: multicellular organismal development; chromatin modification; embryonic cleavage
Research Articles on Dppa3
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Product Notes
The Dppa3 dppa3 (Catalog #AAA9205968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Dppa3 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dppa3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Dppa3 dppa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dppa3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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