Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Zymolyase 100T Enzyme
Zymolyase 100T (Lyticase, Yeast Lytic Enzyme)
Purity
Molecular Biology Grade
Synonyms
Zymolyase 100T; Zymolyase 100T (Lyticase; Yeast Lytic Enzyme); Zymolyase 100T enzyme
Host
Arthrobacter luteus
Purity/Purification
Molecular Biology Grade
Form/Format
Lyophilized powder; 0.06M Phosphate Buffer, pH 7.5
Application Notes
RNase Activity Assay:
Trace RNase activity was detected.
Method: MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5. 10ul of MBS653685 was mixed with 140ul of cCMP (0.1mg/ml in 50mM Tris-HCl buffer, pH 7.5) and the increase of absorbance at 284nm was measured.
Unit Definition:
One RNase A unit is defined as the amount required for hydrolysis of cCMP that results in an increase of 0.001 absorbance units at 284nm per minute.
DNase Activity Assay:
Trace DNase activity was detected.Method:
MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5, at a concentration of 2mg/ml and centrifuged to remove the debris. The supernantant was used for the DNase assay. Reaction mixture (50mM Tris-HCl, pH 7.5, 1mM MnCl2, 50ng/ul lambda DNA-HindIII-digest, 0.4mg/ml MBS653685) was incubated at 30°C for 12 hours, inactivated by heating, and the degradation of lambda DNA was detected by using agarose gel electrophoresis.
Experimental Precautions:
1. Avoid nitrocellulose filters due to non-specific adsorption onto the membrane.
2. If using sterilized MBS653685 in concentrations higher than 0.05%, prepare a 2% solution in buffers containing 5% glucose. Filter the suspension and then dilute with an appropriate buffer.
Trace RNase activity was detected.
Method: MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5. 10ul of MBS653685 was mixed with 140ul of cCMP (0.1mg/ml in 50mM Tris-HCl buffer, pH 7.5) and the increase of absorbance at 284nm was measured.
Unit Definition:
One RNase A unit is defined as the amount required for hydrolysis of cCMP that results in an increase of 0.001 absorbance units at 284nm per minute.
DNase Activity Assay:
Trace DNase activity was detected.Method:
MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5, at a concentration of 2mg/ml and centrifuged to remove the debris. The supernantant was used for the DNase assay. Reaction mixture (50mM Tris-HCl, pH 7.5, 1mM MnCl2, 50ng/ul lambda DNA-HindIII-digest, 0.4mg/ml MBS653685) was incubated at 30°C for 12 hours, inactivated by heating, and the degradation of lambda DNA was detected by using agarose gel electrophoresis.
Experimental Precautions:
1. Avoid nitrocellulose filters due to non-specific adsorption onto the membrane.
2. If using sterilized MBS653685 in concentrations higher than 0.05%, prepare a 2% solution in buffers containing 5% glucose. Filter the suspension and then dilute with an appropriate buffer.
Essential Enzyme
beta-1,3-glucan laminaripentaohydrolase
beta-1,3-glucanase
1.0x10e7 units/g
Protease
1.7x10e4 units/g
Mannanase
6.0x10e4 units/g
RNase/DNase
Trace levels reported
Optimum pH
7.5@35°C, For lysis of viable yeast cells
6.5@45°C, For hydrolysis of yeast glucan
6.5@45°C, For hydrolysis of yeast glucan
Activity
113u/mg
Stable pH
5-10
Heat Stability
Lytic activity lost on incubation at 60ºC for 5 min.
Activators
SH compounds - 2-mercaptoethanol, cysteine or dithiothreitol
Unit Definition
One unit of lytic activity is defined as that amount which indicates 30% decrease in absorbance at 800nm (A800) of the reaction mixture under the conditions indicated in the protocol.
Activators
SH compounds - 2-mercaptoethanol, cysteine or dithiothreitol
Preparation and Storage
Stable for 1 year at 4ºC. About 90% of the lytic activity is lost when stored at 30ºC for 3 months.
Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Testing Data
(Source:Arthrobacter luteus)
Testing Data
(Source:Arthrobacter luteus)
Product Categories/Family for Zymolyase 100T enzyme
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Product Notes
The Zymolyase 100T (Catalog #AAA653685) is an Enzyme produced from Arthrobacter luteus and is intended for research purposes only. The product is available for immediate purchase. RNase Activity Assay: Trace RNase activity was detected. Method: MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5. 10ul of MBS653685 was mixed with 140ul of cCMP (0.1mg/ml in 50mM Tris-HCl buffer, pH 7.5) and the increase of absorbance at 284nm was measured. Unit Definition: One RNase An unit is defined as the amount required for hydrolysis of cCMP that results in an increase of 0.001 absorbance units at 284nm per minute. DNase Activity Assay: Trace DNase activity was detected.Method: MBS653685 was suspended in 50mM Tris-HCl buffer, pH 7.5, at a concentration of 2mg/ml and centrifuged to remove the debris. The supernantant was used for the DNase assay. Reaction mixture (50mM Tris-HCl, pH 7.5, 1mM MnCl2, 50ng/ul lambda DNA-HindIII-digest, 0.4mg/ml MBS653685) was incubated at 30°C for 12 hours, inactivated by heating, and the degradation of lambda DNA was detected by using agarose gel electrophoresis. Experimental Precautions: 1. Avoid nitrocellulose filters due to non-specific adsorption onto the membrane. 2. If using sterilized MBS653685 in concentrations higher than 0.05%, prepare a 2% solution in buffers containing 5% glucose. Filter the suspension and then dilute with an appropriate buffer. Researchers should empirically determine the suitability of the Zymolyase 100T for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zymolyase 100T, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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