Dnmt1 blocking peptide

Dnmt1 Blocking Peptide (Center)

• Gene Names: Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• Synonyms: Dnmt1; Dnmt1 Blocking Peptide (Center); DNA (cytosine-5)-methyltransferase 1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; MMmuI; MCMT; Dnmt; Met1; Uim; Dnmt1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 881-894 of HUMAN Dnmt1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1620

• Cellular Location: Nucleus. Cytoplasm. Note: It is nucleoplasmic through most of the cell cycle and associates with replication foci during S-phase. In germ cells, spermatogonia, preleptotene and leptotene spermatocytes all express high levels of nuclear protein, while the protein is not detected in pachytene spermatocytes, despite the fact they expressed high levels of mRNA. In females, the protein is not detected in non-growing oocytes, in contrast to the growing oocytes. During the growing, the protein is no longer detectable in nuclei but accumulates to very high levels first throughout the cytoplasm. At the time of ovulation, all the protein is cytoplasmic and is actively associated with the oocyte cortex. After fecondation, in the preimplantation embryo, the protein remains cytoplasmic and after implantation, it is exclusively nuclear in all tissue types Isoform 2 is sequestered in the cytoplasm of maturing oocytes and of preimplantation embryos, except for the 8-cell stage, while isoform 1 is exclusively nuclear
• Tissue Location: Isoform 1 is expressed in embryonic stem cells and in somatic tissues. Isoform 2 is expressed in oocytes, preimplantation embryos, testis and in skeletal muscle during myogenesis
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Dnmt1 blocking peptide

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

NCBI and Uniprot Product Information

• NCBI GI #: 20141336
• NCBI GeneID: 13433
• UniProt Accession #: P13864; P97413; Q80ZU3; Q9CSC6; Q9QXX6
• Molecular Weight: 169,996 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1
• NCBI Official Synonym Full Names: DNA methyltransferase (cytosine-5) 1
• NCBI Official Symbol: Dnmt1
• NCBI Official Synonym Symbols: Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: Dnmt1
• UniProt Synonym Gene Names: Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
• UniProt Entry Name: DNMT1_MOUSE

NCBI Description

This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Amino Acid Metabolism - cysteine and methionine; Methyltransferase, DNA; Cell development/differentiation; Methyltransferase; Transcription regulation; EC 2.1.1.37

Cellular Component: cell soma; centric heterochromatin; cytoplasm; heterochromatin; nucleus; protein complex; replication fork

Molecular Function: chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; double-stranded DNA binding; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; methyltransferase activity; protein binding; protein domain specific binding; RNA binding; unmethylated CpG binding; zinc ion binding

Biological Process: cytosine methylation within a CG sequence; DNA methylation; DNA methylation during embryonic development; DNA methylation on cytosine; gene silencing; maintenance of DNA methylation; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K4 methylation; Ras protein signal transduction; regulation of cell proliferation; regulation of gene expression; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; S-adenosylmethionine metabolic process