DCP2 blocking peptide

DCP2 Peptide

Cat. Num. AAA154014

• Gene Names: DCP2; NUDT20
• Applications: Blocking (BL)
• Synonyms: DCP2; DCP2 Peptide; NUDT20; m7GpppN-mRNA hydrolase; Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20; DCP2 decapping enzyme homolog (S. cerevisiae); DCP2 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 420

• Applicable Applications for DCP2 blocking peptide: Blocking (BL)
• Application Notes: DCP2 peptide is used for blocking the activity of DCP2 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 18 amino acids near the carboxy terminus of human DCP2.
• Preparation and Storage: Store DCP2 peptide at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 31542498
• NCBI GeneID: 167227
• NCBI Accession #: NP_689837
• NCBI GenBank Nucleotide #: NM_152624.5
• UniProt Accession #: Q8IU60; Q6P2D4; Q7Z5W5; Q8NBG5; C9J778
• Molecular Weight: 44,443 Da
• NCBI Official Full Name: m7GpppN-mRNA hydrolase isoform 1
• NCBI Official Synonym Full Names: decapping mRNA 2
• NCBI Official Symbol: DCP2
• NCBI Official Synonym Symbols: NUDT20
• NCBI Protein Information: m7GpppN-mRNA hydrolase; hDpc; mRNA-decapping enzyme 2; DCP2 decapping enzyme homolog; nudix (nucleoside diphosphate linked moiety X)-type motif 20
• UniProt Protein Name: m7GpppN-mRNA hydrolase
• Protein Family: mRNA-decapping enzyme
• UniProt Gene Name: DCP2
• UniProt Synonym Gene Names: NUDT20; Nudix motif 20; hDpc
• UniProt Entry Name: DCP2_HUMAN

NCBI Description

The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; EC 3.6.1.62; Hydrolase

Chromosomal Location of Human Ortholog: 5q22.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cell junction; cytosol

Molecular Function: m7G(5')pppN diphosphatase activity; protein binding; exoribonuclease activity, producing 5'-phosphomonoesters; RNA binding; manganese ion binding

Biological Process: mRNA catabolic process; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA catabolic process, deadenylation-dependent decay