CYP4F8 blocking peptide

CYP4F8 Antibody (C-term) Blocking Peptide

• Gene Names: CYP4F8; CPF8; CYPIVF8
• Synonyms: CYP4F8; CYP4F8 Antibody (C-term) Blocking Peptide; Cytochrome P450 4F8; CYPIVF8; CYP4F8 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CYP4F8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 520

• Cellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein Microsome membrane
• Tissue Location: Expressed in the epithelium of seminal vesicles, in renal cortex, in adult and fetal liver, in epidermis, in corneal epithelium, in sweat glands, hair follicles, epithelial linings of the ampulla of vas deferens and of the stomach and small intestine, as well as in the transitional epithelium of the bladder and ureter (at protein level). In the epidermis, expressed from the basal cell to the granular cell layers. In the corneal epithelium, expressed in all cell layers. Also detected in prostate. Up-regulated in the epidermis of psoriatic lesions
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CYP4F8 blocking peptide

Hydroxylates arachidonic acid (20:4n-6) to (18R)- hydroxyarachidonate. Shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. Catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3- hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). Catalyzes hydroxylation of PGI2 and carbaprostacyclin.

NCBI and Uniprot Product Information

• NCBI GI #: 10719963
• NCBI GeneID: 11283
• UniProt Accession #: P98187
• Molecular Weight: 59,995 Da
• NCBI Official Full Name: Cytochrome P450 4F8
• NCBI Official Synonym Full Names: cytochrome P450 family 4 subfamily F member 8
• NCBI Official Symbol: CYP4F8
• NCBI Official Synonym Symbols: CPF8; CYPIVF8
• NCBI Protein Information: cytochrome P450 4F8
• UniProt Protein Name: Cytochrome P450 4F8
• Protein Family: Cytochrome
• UniProt Gene Name: CYP4F8
• UniProt Entry Name: CP4F8_HUMAN

NCBI Description

This gene, CYP4F8, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and functions as a 19-hydroxylase of prostaglandins in seminal vesicles. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F3, is approximately 18 kb away. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP4F8: Hydroxylates arachidonic acid (20:4n-6) to (18R)- hydroxyarachidonate. Shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. Catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3- hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). Catalyzes hydrxylation of PGI2 and carbaprostacyclin. Belongs to the cytochrome P450 family.

Protein type: Membrane protein, integral; EC 1.14.14.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: endoplasmic reticulum membrane

Molecular Function: alkane 1-monooxygenase activity; oxygen binding

Biological Process: icosanoid metabolic process; prostaglandin metabolic process