Ctr9 blocking peptide

Mouse Ctr9 Blocking Peptide (C-term)

• Gene Names: Ctr9; Tsp; Tsbp; Sh2bp1; AA409336; mKIAA0155
• Synonyms: Ctr9; Mouse Ctr9 Blocking Peptide (C-term); RNA polymerase-associated protein CTR9 homolog; SH2 domain-binding protein 1; Tetratricopeptide repeat-containing; SH2-binding phosphoprotein of 150 kDa; TPR-containing; p150TSP; Kiaa0155; Sh2bp1; Ctr9 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 1022-1036 of HUMAN Ctr9
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1173

• Cellular Location: Nucleus speckle
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Ctr9 blocking peptide

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys- 4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.

NCBI and Uniprot Product Information

• NCBI GI #: 91208163
• NCBI GeneID: 22083
• UniProt Accession #: Q62018; Q3UFF5; Q3UY40; Q66JX4; Q7TPS6; Q8BND9; Q8BRD1; Q8C9W7; Q8C9Y3; Q8CHI1
• Molecular Weight: 123,864 Da
• NCBI Official Full Name: RNA polymerase-associated protein CTR9 homolog
• NCBI Official Synonym Full Names: CTR9 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: Ctr9
• NCBI Official Synonym Symbols: Tsp; Tsbp; Sh2bp1; AA409336; mKIAA0155
• NCBI Protein Information: RNA polymerase-associated protein CTR9 homolog
• UniProt Protein Name: RNA polymerase-associated protein CTR9 homolog
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: Ctr9
• UniProt Synonym Gene Names: Kiaa0155; Sh2bp1; TPR-containing, SH2-binding phosphoprotein of 150 kDa; p150TSP
• UniProt Entry Name: CTR9_MOUSE

Uniprot Description

SH2BP1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.

Protein type: Transcription regulation

Cellular Component: nucleus

Molecular Function: protein binding; SH2 domain binding

Biological Process: blastocyst growth; endodermal cell fate commitment; histone H2B ubiquitination; histone monoubiquitination; inner cell mass cell differentiation; JAK-STAT cascade; negative regulation of myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; stem cell maintenance; trophectodermal cell differentiation