CTDP1 blocking peptide

CTDP1 Antibody (N-term) Blocking Peptide

• Gene Names: CTDP1; FCP1; CCFDN
• Synonyms: CTDP1; CTDP1 Antibody (N-term) Blocking Peptide; RNA polymerase II subunit A C-terminal domain phosphatase; TFIIF-associating CTD phosphatase; FCP1; CTDP1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human CTDP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 961

• Cellular Location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Midbody. Note: Found at centrosomes in prometaphase, at spindle and spindle poles in metaphase and at spindle midzone and midbody in anaphase and telophase-G1 respectively
• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CTDP1 blocking peptide

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.

NCBI and Uniprot Product Information

• NCBI GI #: 327478586
• NCBI GeneID: 9150
• UniProt Accession #: Q9Y5B0; Q7Z644; Q96BZ1; Q9Y6F5; A8MY97
• Molecular Weight: 93,485 Da
• NCBI Official Full Name: RNA polymerase II subunit A C-terminal domain phosphatase
• NCBI Official Synonym Full Names: CTD phosphatase subunit 1
• NCBI Official Symbol: CTDP1
• NCBI Official Synonym Symbols: FCP1; CCFDN
• NCBI Protein Information: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Protein Name: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Gene Name: CTDP1
• UniProt Synonym Gene Names: FCP1
• UniProt Entry Name: CTDP1_HUMAN

NCBI Description

This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

CTDP1: a phospho-serine/threonine protein phosphatase which interacts with the carboxy-terminus of transcription initiation factor TFIIF-alpha, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. Its phosphorylation increases binding to TFIIF-alpha, affecting transcription elongation. Alternative splicing results in two different isoforms.

Protein type: Transcription initiation complex; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: actin cytoskeleton; centrosome; DNA-directed RNA polymerase II, holoenzyme; midbody; nucleoplasm; spindle; spindle midzone; spindle pole

Molecular Function: CTD phosphatase activity; phosphoprotein phosphatase activity; protein binding

Biological Process: exit from mitosis; positive regulation of viral transcription; protein amino acid dephosphorylation; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Congenital Cataracts, Facial Dysmorphism, And Neuropathy