Human CTD Phosphatase 1 ELISA Kit | CTDP1 elisa kit

Human CTD Phosphatase 1 ELISA Kit

Cat. Num. AAA726389

• Gene Names: CTDP1; FCP1; CCFDN
• Reactivity: Human
• Synonyms: CTD Phosphatase 1; Human CTD Phosphatase 1 ELISA Kit; CTDP1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTDP1 elisa kit

Intended Uses: This CPN IGM ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCPN IGM in the sample, thisCPN IGM ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCPN IGM concentration. The concentration ofCPN IGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This CPN IGM enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCPN IGM. Standards or samples are then added to the microtiter plate wells andCPN IGM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCPN IGM present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCPN IGM are added to each well to "sandwich" theCPN IGM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCPN IGM and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 30962890
• NCBI GeneID: 9150
• Molecular Weight: 93,485 Da
• NCBI Official Full Name: CTDP1 protein, partial
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
• NCBI Official Symbol: CTDP1
• NCBI Official Synonym Symbols: FCP1; CCFDN
• NCBI Protein Information: RNA polymerase II subunit A C-terminal domain phosphatase; serine phosphatase FCP1a; TFIIF-associating CTD phosphatase 1; CTD of POLR2A, phosphatase of, subunit 1; transcription factor IIF-associating CTD phosphatase 1
• UniProt Protein Name: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Gene Name: CTDP1
• UniProt Synonym Gene Names: FCP1
• UniProt Entry Name: CTDP1_HUMAN

NCBI Description

This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

CTDP1: a phospho-serine/threonine protein phosphatase which interacts with the carboxy-terminus of transcription initiation factor TFIIF-alpha, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. Its phosphorylation increases binding to TFIIF-alpha, affecting transcription elongation. Alternative splicing results in two different isoforms.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Transcription initiation complex

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; spindle; midbody; spindle midzone; actin cytoskeleton

Molecular Function: DNA-directed RNA polymerase activity; CTD phosphatase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; cell division; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; protein amino acid dephosphorylation; exit from mitosis

Disease: Congenital Cataracts, Facial Dysmorphism, And Neuropathy