Csk blocking peptide

Mouse Csk Blocking Peptide (C-term)

• Gene Names: Csk; p50CSK; AW212630
• Synonyms: Csk; Mouse Csk Blocking Peptide (C-term); Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase MPK-2; p50CSK; Csk blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 398-411 of HUMAN Csk
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 450

• Cellular Location: Cytoplasm. Cell membrane. Note: Mainly cytoplasmic, also present in lipid rafts
• Tissue Location: Ubiquitous, but most abundant in thymus and spleen, as well as in neonatal brain
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Csk blocking peptide

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 341940406
• NCBI GeneID: 12988
• UniProt Accession #: P41241; Q03143; Q80WU4; Q8VCW1
• Molecular Weight: 50,716 Da
• NCBI Official Full Name: Tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: Csk
• NCBI Official Synonym Symbols: p50CSK; AW212630
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: Csk
• UniProt Entry Name: CSK_MOUSE

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Csk family

Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane; intercellular junction; lipid raft; plasma membrane

Molecular Function: ATP binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding; transferase activity

Biological Process: cell differentiation; cell migration; central nervous system development; innate immune response; negative regulation of bone resorption; negative regulation of cell proliferation; negative regulation of Golgi to plasma membrane protein transport; negative regulation of interleukin-6 production; negative regulation of kinase activity; negative regulation of phagocytosis; positive regulation of MAP kinase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cytokine production; regulation of T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway