Rabbit anti-Mouse CSK Polyclonal Antibody | anti-CSK antibody

Anti-CSK Antibody, Rabbit Polyclonal

Cat. Num. AAA8112145

• Gene Names: Csk; AW212630
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CSK; Polyclonal Antibody; Anti-CSK Antibody; Rabbit Polyclonal; C-Src Kinase/CSK Antibody; Rabbit PAb; Antigen Affinity Purified; c-src tyrosine kinase; Anti-AW212630 Antibody; Anti-p50CSK Antibody; anti-CSK antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse C-Src Kinase/CSK
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CSK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Mouse C-Src Kinase/CSK protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse C-Src Kinase/CSK. C-Src Kinase/CSK specific IgG was purified by Mouse C-Src Kinase/CSK affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CSK rabbit polyclonal antibody at 1:500 dilution.Lane A: Jurkat Whole Cell LysateLane B: Mouse spleen tissue lysate Lane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:51 kDaObserved band size:51 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CSK antibody

The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.

References

Product Categories/Family for anti-CSK antibody

EGFR Signaling Pathway

References

Brauninger A. et al., 1992, Gene. 110: 205-11. Sondhi D. et al., 1999, Biochemistry. 38 (34): 11147-55. Ogawa A. et al., 2002, J Biol Chem. 277 (17): 14351-4. Cole PA. et al., 2003, Curr Opin Chem Biol. 7 (5): 580-5. Baumeister U. et al., 2005, EMBO J. 24 (9): 1686-95.

NCBI and Uniprot Product Information

• NCBI GI #: 31560712
• NCBI GeneID: 12988
• NCBI Accession #: NP_031809.2
• NCBI GenBank Nucleotide #: NM_007783.2
• UniProt Accession #: P41241; Q03143; Q80WU4; Q8VCW1
• Molecular Weight: 50,716 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: Csk
• NCBI Official Synonym Symbols: AW212630
• NCBI Protein Information: tyrosine-protein kinase CSK; p50CSK; C-Src kinase; protein-tyrosine kinase MPK-2
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: Csk
• UniProt Entry Name: CSK_MOUSE