Testing Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC )
Mouse anti-Human CD324 Monoclonal Antibody | anti-CD324 antibody
Mouse Anti Human CD324: RPE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Testing Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC )
Testing Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC )
Testing Data
(Surface Staining of HT-29 cells with Mouse anti Human CD324:FITC )
Testing Data
(Surface Staining of HT-29 cells with Mouse anti Human CD324:FITC )
Testing Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE )
Testing Data
(Surface Staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE )
The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions (Gloushankova et al. 2017).
The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells (Mendonsa et al. 2018).
This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Uniprot Description
CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: apical junction complex; internal side of plasma membrane; focal adhesion; cell surface; lateral loop; basolateral plasma membrane; extracellular region; integral to membrane; trans-Golgi network; catenin complex; actin cytoskeleton; cell-cell adherens junction; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane
Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; cell adhesion molecule binding; ankyrin binding; glycoprotein binding; protein phosphatase binding
Biological Process: response to drug; intercellular junction assembly and maintenance; extracellular matrix organization and biogenesis; regulation of immune response; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; regulation of caspase activity; trophectodermal cell differentiation; regulation of water loss via skin; extracellular matrix disassembly; cell-cell adhesion; synaptogenesis; sensory perception of sound; pituitary gland development; calcium-dependent cell-cell adhesion; protein metabolic process; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; protein homooligomerization; neurite development; negative regulation of epithelial cell proliferation; cell structure disassembly during apoptosis
Disease: Gastric Cancer, Hereditary Diffuse; Prostate Cancer; Breast Cancer; Ovarian Cancer; Endometrial Cancer
Research Articles on CD324
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Product Notes
The CD324 cdh1 (Catalog #AAA225802) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD324: RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD324 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max. Dilution: Neat Use 10ul of the undiluted reagent to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD324 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD324, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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