CLCN3 blocking peptide

CLCN3 Antibody (Center) Blocking Peptide

• Gene Names: CLCN3; CLC3; ClC-3
• Synonyms: CLCN3; CLCN3 Antibody (Center) Blocking Peptide; H(+)/Cl(-) exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3; CLCN3 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 818

• Cellular Location: Isoform 1: Membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein Late endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Note: Isoform 1 is localized mainly in early and late endosomes
• Tissue Location: Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CLCN3 blocking peptide

Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.

NCBI and Uniprot Product Information

• NCBI GI #: 226693513
• NCBI GeneID: 1182
• UniProt Accession #: P51790; O14918; Q86Z21; B7Z932; B9EGJ9; D3DP34; E9PB97
• Molecular Weight: 88,781 Da
• NCBI Official Full Name: H(+)/Cl(-) exchange transporter 3
• NCBI Official Synonym Full Names: chloride voltage-gated channel 3
• NCBI Official Symbol: CLCN3
• NCBI Official Synonym Symbols: CLC3; ClC-3
• NCBI Protein Information: H(+)/Cl(-) exchange transporter 3
• UniProt Protein Name: H(+)/Cl(-) exchange transporter 3
• Protein Family: H(+)/Cl(-) exchange transporter
• UniProt Gene Name: CLCN3
• UniProt Synonym Gene Names: ClC-3
• UniProt Entry Name: CLCN3_HUMAN

NCBI Description

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass; Channel, chloride; Vesicle; Transporter

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: cytoplasmic vesicle; early endosome; external side of plasma membrane; Golgi apparatus; integral to membrane; late endosome; membrane; phagocytic vesicle; plasma membrane; secretory granule; specific granule; synaptic vesicle; vesicle membrane

Molecular Function: chloride channel activity; PDZ domain binding; protein binding; protein heterodimerization activity

Biological Process: endosomal lumen acidification; negative regulation of cell volume; regulation of pH; transport