CENPE blocking peptide

CENPE Antibody (C-term) Blocking Peptide

• Gene Names: CENPE; KIF10; CENP-E; MCPH13; PPP1R61
• Synonyms: CENPE; CENPE Antibody (C-term) Blocking Peptide; Centromere-associated protein E; Centromere protein E; CENP-E; Kinesin-related protein CENPE; CENPE blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 2701

• Cellular Location: Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CENPE blocking peptide

Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 160358869
• NCBI GeneID: 1062
• UniProt Accession #: Q02224; Q4LE75; A6NKY9; A8K2U7
• Molecular Weight: 301,789 Da
• NCBI Official Full Name: Centromere-associated protein E
• NCBI Official Synonym Full Names: centromere protein E
• NCBI Official Symbol: CENPE
• NCBI Official Synonym Symbols: KIF10; CENP-E; MCPH13; PPP1R61
• NCBI Protein Information: centromere-associated protein E
• UniProt Protein Name: Centromere-associated protein E
• Protein Family: Centromere-associated protein
• UniProt Gene Name: CENPE
• UniProt Synonym Gene Names: CENP-E
• UniProt Entry Name: CENPE_HUMAN

NCBI Description

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: chromosome; chromosome, pericentric region; cytoplasm; cytosol; kinesin complex; kinetochore; membrane; microtubule; microtubule cytoskeleton; midbody; nucleus; outer kinetochore of condensed chromosome

Molecular Function: ATPase activity; kinetochore binding; microtubule motor activity; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; attachment of spindle microtubules to kinetochore during mitosis; chromosome segregation; microtubule-based movement; mitotic cell cycle; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; positive regulation of protein kinase activity; regulation of mitotic metaphase/anaphase transition; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion

Disease: Microcephaly 13, Primary, Autosomal Recessive