Rabbit anti-Human CENPE Polyclonal Antibody | anti-CENPE antibody

CENPE Rabbit pAb

Cat. Num. AAA4756227

• Gene Names: CENPE; KIF10; CENP-E; PPP1R61
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: CENPE; Polyclonal Antibody; CENPE Rabbit pAb; Centromere-associated protein E; centromere protein E; KIF10; CENP-E; MCPH13; PPP1R61; anti-CENPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-CENPE antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:5000
• Immunogen: A synthetic peptide of human CENPE
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of CENPE in Jurkat lysates using CENPE antibody.Predicted band size:316kDa.Observed band size:316kDa.)

Related Product Information for anti-CENPE antibody

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:7889940, PubMed:23891108, PubMed:25395579). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss.
Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

Product Categories/Family for anti-CENPE antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 71061468
• NCBI GeneID: 1062
• NCBI Accession #: NP_001804.2
• NCBI GenBank Nucleotide #: NM_001813.2
• UniProt Accession #: Q02224; Q4LE75; A6NKY9; A8K2U7
• Molecular Weight: 316,415 Da
• NCBI Official Full Name: centromere-associated protein E
• NCBI Official Synonym Full Names: centromere protein E, 312kDa
• NCBI Official Symbol: CENPE
• NCBI Official Synonym Symbols: KIF10; CENP-E; PPP1R61
• NCBI Protein Information: centromere-associated protein E; kinesin family member 10; kinesin-related protein CENPE; Centromere autoantigen E (312kD); protein phosphatase 1, regulatory subunit 61
• UniProt Protein Name: Centromere-associated protein E
• Protein Family: Centromere-associated protein
• UniProt Gene Name: CENPE
• UniProt Synonym Gene Names: CENP-E
• UniProt Entry Name: CENPE_HUMAN

NCBI Description

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: kinetochore; microtubule cytoskeleton; kinesin complex; microtubule; membrane; cytoplasm; outer kinetochore of condensed chromosome; chromosome; midbody; cytosol; nucleus; chromosome, pericentric region

Molecular Function: kinetochore binding; protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: kinetochore assembly; metabolic process; multicellular organismal development; regulation of mitotic metaphase/anaphase transition; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; microtubule-based movement; chromosome segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; positive regulation of protein kinase activity; mitotic cell cycle; blood coagulation

Disease: Microcephaly 13, Primary, Autosomal Recessive