ATP6V1A blocking peptide

ATP6V1A Blocking Peptide

Cat. Num. AAA9621058

• Gene Names: ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ATP6V1A; ATP6V1A Blocking Peptide; 70kDa subunit; ATP6A1; ATP6V1A1; ATPase H+ transporting lysosomal subunit A1; ATPase H+ transporting; lysosomal 70kDa V1 subunit A; H(+) transporting two sector ATPase subunit A; H+ transporting ATPase chain A vacuolar (VA68 type); HO68; OTTHUMP00000214746; V ATPase 69 kDa subunit 1; V ATPase A subunit 1; V ATPase subunit A 1; V-ATPase 69 kDa subunit; V-ATPase subunit A; V-type proton ATPase catalytic subunit A; VA68; Vacuolar ATP synthase catalytic subunit A ubiquitous isoform; Vacuolar ATPase isoform VA68; Vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha; VATA_HUMAN; Vma1; VPP2; ATP6V1A blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ATP6V1A blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATP6V1A blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 19913424
• NCBI GeneID: 523
• NCBI Accession #: NP_001681.2
• NCBI GenBank Nucleotide #: NM_001690.3
• UniProt Accession #: P38606; Q53YD9; Q96DY6; Q9UHY3; B2RBR8; B7Z1R5; D3DN75
• Molecular Weight: 64,736 Da
• NCBI Official Full Name: V-type proton ATPase catalytic subunit A
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• NCBI Official Symbol: ATP6V1A
• NCBI Official Synonym Symbols: HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
• NCBI Protein Information: V-type proton ATPase catalytic subunit A; V-ATPase subunit A; V-ATPase A subunit 1; V-ATPase 69 kDa subunit 1; vacuolar ATPase isoform VA68; vacuolar proton pump subunit alpha; vacuolar proton pump alpha subunit 1; ATPase, H+ transporting, lysosomal, subu
• UniProt Protein Name: V-type proton ATPase catalytic subunit A
• UniProt Gene Name: ATP6V1A
• UniProt Synonym Gene Names: ATP6A1; ATP6V1A1; VPP2; V-ATPase subunit A
• UniProt Entry Name: VATA_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1A: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: microvillus; mitochondrion; integral to plasma membrane; lysosomal membrane; apical plasma membrane; plasma membrane; proton-transporting two-sector ATPase complex; cytosol

Molecular Function: hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding

Biological Process: ATP metabolic process; interaction with host; cellular iron ion homeostasis; transport; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport