Survivin ELISA Kit | BIRC5 elisa kit

Monkey primate Survivin PicoKine ELISA Kit

Cat. Num. AAA1751066

• Gene Names: BIRC5; API4; EPR-1
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: Survivin; Monkey primate Survivin PicoKine ELISA Kit; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; API4; IAP4; BIRC5 elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Monkey primate Survivin
• Sequence Length: 74

• Applicable Applications for BIRC5 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BIRC5 elisa kit

Principle of the Assay: 215 Monkey primate Survivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate Survivin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: M1-D142) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate Survivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate Survivin amount of sample captured in plate.

Background: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common monkey primate cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non- Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant monkey primate Survivin with the molecular mass of 34 KDa.

Product Categories/Family for BIRC5 elisa kit

Cell Biology; Apoptosis; Intracellular; Survivin/IAPS; Epigenetics and Nuclear Signaling; Chromosome Structure; Chromosome; Scaffold Proteins; Cell Cycle; Nuclear; Transcription; Transcription Factors; Cell Biology; Cell Division; Other Cell Division Anti

NCBI and Uniprot Product Information

• NCBI GI #: 59859880
• NCBI GeneID: 332
• NCBI Accession #: NP_001012270.1
• NCBI GenBank Nucleotide #: NM_001012270.1
• UniProt Accession #: O15392; Q2I3N8; Q4VGX0; Q53F61; Q5MGC6; Q6FHL2; Q75SP2; Q9P2W8; A2SUH6; B2R4R1
• Molecular Weight: 16389 MW
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5 isoform 2
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 5
• NCBI Official Symbol: BIRC5
• NCBI Official Synonym Symbols: API4; EPR-1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC5
• UniProt Synonym Gene Names: API4; IAP4

NCBI Description

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).