BCHE / Butyrylcholinesterase Recombinant Protein | BCHE recombinant protein

Recombinant Mouse BCHE / Butyrylcholinesterase Protein (His tag)

Cat. Num. AAA2546991

• Gene Names: Bche; C730038G20Rik
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: BCHE / Butyrylcholinesterase; Recombinant Mouse BCHE / Butyrylcholinesterase Protein (His tag); C730038G20Rik; MGC107651; Bche; BCHE recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5
• Sequence Length: 603

• Application Notes: The secreted recombinant mouse BCHE consists of 587 amino acids and has a calculated molecular mass of 66.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BCHE recombinant protein

Background: Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.

Description: A DNA sequence encoding the mouse BCHE (NP_033868.3) (Met 1-Leu 603) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 124487350
• NCBI GeneID: 12038
• NCBI Accession #: NP_033868.3
• NCBI GenBank Nucleotide #: NM_009738.3
• UniProt Accession #: Q03311; Q543J3
• Molecular Weight: 68,462 Da
• NCBI Official Full Name: cholinesterase
• NCBI Official Synonym Full Names: butyrylcholinesterase
• NCBI Official Symbol: Bche
• NCBI Official Synonym Symbols: C730038G20Rik
• NCBI Protein Information: cholinesterase
• UniProt Protein Name: Cholinesterase
• Protein Family: Cholinesterase
• UniProt Gene Name: Bche

Uniprot Description

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: EC 3.1.1.8; Hydrolase; Nuclear envelope; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3|3 E3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular space; membrane; nuclear envelope lumen

Molecular Function: acetylcholinesterase activity; choline binding; cholinesterase activity

Biological Process: choline metabolic process; learning; negative regulation of cell proliferation; negative regulation of synaptic transmission; neuroblast differentiation; response to alkaloid; response to drug; response to folic acid; response to glucocorticoid stimulus; response to nutrient