CD31 Recombinant Protein | PECAM1 recombinant protein

CD31 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122725

• Gene Names: Bche; C730038G20Rik
• Purity: >93% as determined by SDS-PAGE
• Synonyms: CD31; CD31 Protein; Mouse; Recombinant (His Tag); Mouse CD31/PECAM-1 Protein (His Tag); C85791 Protein; Cd31 Protein; Pecam Protein; PECAM-1 Protein; platelet/endothelial cell adhesion molecule 1; PECAM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >93% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA
• Sequence: Met1-Lys590

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PECAM1 (NP_033868.3) extracellular domain (Met 1-Lys 590) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PECAM1 recombinant protein

Background: The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin alphavbeta3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Product Categories/Family for PECAM1 recombinant protein

Hemangioblast Markers ITIM/ITAM Immunoreceptors and Related Molecules

References

Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.

NCBI and Uniprot Product Information

• NCBI GI #: 124487350
• NCBI GeneID: 12038
• NCBI Accession #: NP_033868.3
• NCBI GenBank Nucleotide #: NM_009738.3
• UniProt Accession #: Q03311; Q543J3
• Molecular Weight: 68,462 Da
• NCBI Official Full Name: cholinesterase
• NCBI Official Synonym Full Names: butyrylcholinesterase
• NCBI Official Symbol: Bche
• NCBI Official Synonym Symbols: C730038G20Rik
• NCBI Protein Information: cholinesterase
• UniProt Protein Name: Cholinesterase
• Protein Family: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: Bche
• UniProt Entry Name: CHLE_MOUSE

Uniprot Description

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: Hydrolase; Nuclear envelope; Secreted; EC 3.1.1.8; Secreted, signal peptide

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; extracellular space; membrane; nuclear envelope lumen

Molecular Function: 1-oxa-2-oxocycloheptane lactonase activity; 3,4-dihydrocoumarin hydrolase activity; acetylcholinesterase activity; all-trans-retinyl-palmitate hydrolase activity; butyrolactone hydrolase activity; choline binding; cholinesterase activity; hydrolase activity; identical protein binding; lipase activity; phospholipase activity; prenylcysteine methylesterase activity; retinyl-palmitate esterase activity; sulfolactone hydrolase activity

Biological Process: choline metabolic process; learning; negative regulation of cell proliferation; negative regulation of synaptic transmission; neuroblast differentiation; response to alkaloid; response to drug; response to folic acid; response to glucocorticoid stimulus; response to nutrient