BCCIP recombinant protein

BCCIP, 1-314aa, Human, His tag, E Coli

Cat. Num. AAA205525

• Gene Names: BCCIP; TOK1; TOK-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: BCCIP; 1-314aa; Human; His tag; E Coli; BRCA2 and CDKN1A interacting protein; TOK-1; TOK1; BCCIP recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMASRS KRRAVESGVP QPPDPPVQRD EEEEKEVENE DEDDDDSDKE KDEEDEVIDE EVNIEFEAYS LSDNDYDGIK KLLQQLFLKA PVNTAELTDL LIQQNHIGSV IKQTDVSEDS NDDMDEDEVF GFISLLNLTE RKGTQCVEQI QELVLRFCEK NCEKSMVEQL DKFLNDTTKP VGLLLSERFI NVPPQIALPM YQQLQKELAG AHRTNKPCGK CYFYLLISKT FVEAGKNNSK KKPSNKKKAA LMFANAEEEF FYEKAILKFN YSVQEESDTC LGGKWSFDDV PMTPLRTVML IPGDKMNEIM DKLKEYLSV
• Sequence Length: 314

• Applicable Applications for BCCIP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BCCIP recombinant protein

BCCIP was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Recombinant human BCCIP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for BCCIP recombinant protein

Cell Cycle

References

Luedeke,M., et al. (2009) Cancer Epidemiol. Biomarkers Prev. 18 (11), 3030-3035; Fan,J., et al. (2009) Cell Cycle 8 (18), 3019-3024;

NCBI and Uniprot Product Information

• NCBI GI #: 17402871
• NCBI GeneID: 56647
• NCBI Accession #: NP_510868
• NCBI GenBank Nucleotide #: NM_078468.2
• UniProt Accession #: Q9P287; Q8ND15; Q96GC4; Q9P288; B3KP45
• Molecular Weight: 38.6kDa (339aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: BRCA2 and CDKN1A-interacting protein isoform BCCIPbeta
• NCBI Official Synonym Full Names: BRCA2 and CDKN1A interacting protein
• NCBI Official Symbol: BCCIP
• NCBI Official Synonym Symbols: TOK1; TOK-1
• NCBI Protein Information: BRCA2 and CDKN1A-interacting protein
• UniProt Protein Name: BRCA2 and CDKN1A-interacting protein
• Protein Family: BRCA2 and CDKN1A-interacting protein
• UniProt Gene Name: BCCIP
• UniProt Synonym Gene Names: TOK1

NCBI Description

This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342). Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta (PubMed:28394342). May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).