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BBOX1 recombinant protein
BBOX1 Protein, Human, Recombinant (His & GST Tag)
Gene Names
BBOX1; BBH; BBOX; G-BBH; gamma-BBH
Purity
>85% as determined by SDS-PAGE
Synonyms
BBOX1; BBOX1 Protein; Human; Recombinant (His & GST Tag); Human BBOX1/Gamma-BBH Protein (His & GST Tag); BBH Protein; BBOX Protein; G-BBH Protein; gamma-BBH Protein; butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1; BBOX1 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Asn387
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met
Tag
N-GST & His
Protein Construction
A DNA sequence encoding the human BBOX1 (O75936)(Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for BBOX1 recombinant protein
Background: BBOX1, also known as gamma-BBH, belongs to thegamma-BBH/TMLD family. It is highly expressed in kidney and moderately expressed in liver. BBOX1 catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. BBOX1 is an inhibition target for mildronate which can be used to treatanginaandmyocardial infarction. Mildronate may also be beneficial for the treatment ofneurological disorder,diabetes, andseizures andalcohol intoxication.
References
Vaz FM. et al., 1998, Biochem Biophys Res Commun. 250 (2): 506-10. Paul HS. et al., 1992, Eur J Biochem. 203 (3): 599-605. Dawany NB. et al., 2011, Int J Cancer. 128 (12): 2881-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
387
NCBI Official Full Name
Gamma-butyrobetaine dioxygenase
NCBI Official Synonym Full Names
butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
NCBI Official Symbol
BBOX1
NCBI Official Synonym Symbols
BBH; BBOX; G-BBH; gamma-BBH
NCBI Protein Information
gamma-butyrobetaine dioxygenase; gamma-butyrobetaine hydroxylase; gamma-butyrobetaine,2-oxoglutarate dioxygenase 1
UniProt Protein Name
Gamma-butyrobetaine dioxygenase
Protein Family
UniProt Gene Name
BBOX1
UniProt Synonym Gene Names
BBH; BBOX; Gamma-BBH
UniProt Entry Name
BODG_HUMAN
NCBI Description
This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]
Uniprot Description
BBOX1: Catalyzes the formation of L-carnitine from gamma- butyrobetaine. Belongs to the gamma-BBH/TMLD family.
Protein type: EC 1.14.11.1; Oxidoreductase; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 11p14.2
Cellular Component: mitochondrion; cytosol
Molecular Function: gamma-butyrobetaine dioxygenase activity; zinc ion binding; iron ion binding
Biological Process: carnitine biosynthetic process
Research Articles on BBOX1
Similar Products
Product Notes
The BBOX1 bbox1 (Catalog #AAA8121932) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Asn38 7. It is sometimes possible for the material contained within the vial of "BBOX1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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