Casein Kinase 1 alpha Cell Lysate | CSNK1A1 cell lysate
Human Casein Kinase 1 alpha Baculovirus-Insect cells Overexpression Lysate
Gene Names
CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
Applications
Western Blot
Synonyms
Casein Kinase 1 alpha; Human Casein Kinase 1 alpha Baculovirus-Insect cells Overexpression Lysate; Human CSNK1A1/CKI-alpha/CK1 Insect Cell Lysate (WB positive control); casein kinase 1; alpha 1; Human CK1 Overexpression Lysate; Human CK1a Overexpression Lysate; Human CKIa Overexpression Lysate; Human HEL-S-77p Overexpression Lysate; Human HLCDGP1 Overexpression Lysate; Human PRO2975 Overexpression Lysate; CSNK1A1 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
561aa
Sequence
Met1-Phe337
Applicable Applications for CSNK1A1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CSNK1A1 cell lysate
Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.
This Human Casein Kinase 1 alpha overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Casein Kinase 1 alpha protein from the overexpression lysate was verified.
This Human Casein Kinase 1 alpha overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Casein Kinase 1 alpha protein from the overexpression lysate was verified.
Product Categories/Family for CSNK1A1 cell lysate
References
Dubois, et al., 2002, FEBS Lett. (Netherlands) 517 (1-3): 167-71. Dubois, T et al., 2001, J. Biol. Chem.(United States) 276 (22): 18757-64. Zhang, Yi et al., 2002, J. Biol. Chem. 277(20): 17706-12. Wang, Z. et al., 2010, Med Sci Monit.16 (8):CR357-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,915 Da
NCBI Official Full Name
casein kinase I isoform alpha isoform 1
NCBI Official Synonym Full Names
casein kinase 1 alpha 1
NCBI Official Symbol
CSNK1A1
NCBI Official Synonym Symbols
CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
NCBI Protein Information
casein kinase I isoform alpha
UniProt Protein Name
Casein kinase I isoform alpha
Protein Family
UniProt Gene Name
CSNK1A1
UniProt Synonym Gene Names
CKI-alpha
UniProt Entry Name
KC1A_HUMAN
Uniprot Description
CK1A: an ubiquitous protein kinase of the CK1 family. Participates in Wnt signaling. Phosphorylates beta catenin, marking it for beta-TRCp-mediated ubiquitinylation and proteasomal degradation.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CK1; EC 2.7.11.1; CK1 group; CK1 family
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: centrosome; membrane; cytoplasm; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: mitosis; regulation of cell shape; peptidyl-serine phosphorylation; cell surface receptor linked signal transduction; Wnt receptor signaling pathway; cell division; signal transduction; protein amino acid phosphorylation; phagocytosis
Research Articles on CSNK1A1
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Product Notes
The CSNK1A1 csnk1a1 (Catalog #AAA8114018) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 561aa. AAA Biotech's Casein Kinase 1 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CSNK1A1 csnk1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Phe33 7. It is sometimes possible for the material contained within the vial of "Casein Kinase 1 alpha, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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