CSNK1A1 / CKI-alpha / CK1 Recombinant Protein | CSNK1A1 recombinant protein

Recombinant Human CSNK1A1 / CKI-alpha / CK1 Protein (GST tag)

Cat. Num. AAA2545100

• Gene Names: CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CSNK1A1 / CKI-alpha / CK1; Recombinant Human CSNK1A1 / CKI-alpha / CK1 Protein (GST tag); CK1; HLCDGP1; PRO2975; CSNK1A1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4
• Sequence Length: 365

• Application Notes: The recombinant human CSNK1A1/GST chimera consists of 561 amino acids and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CSNK1A1 recombinant protein

Background: Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.

Description: A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 68303575
• NCBI GeneID: 1452
• NCBI Accession #: NP_001020276.1
• NCBI GenBank Nucleotide #: NM_001025105.2
• UniProt Accession #: P48729; Q4JJA0; Q5U046; Q5U047; Q6FGA2; Q71TU5; Q96HD2; Q9UDK3; D3DQG0; D3DQG1
• Molecular Weight: 37,567 Da
• NCBI Official Full Name: casein kinase I isoform alpha isoform 1
• NCBI Official Synonym Full Names: casein kinase 1 alpha 1
• NCBI Official Symbol: CSNK1A1
• NCBI Official Synonym Symbols: CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
• NCBI Protein Information: casein kinase I isoform alpha
• UniProt Protein Name: Casein kinase I isoform alpha
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1A1
• UniProt Synonym Gene Names: CKI-alpha

Uniprot Description

CK1A: an ubiquitous protein kinase of the CK1 family. Participates in Wnt signaling. Phosphorylates beta catenin, marking it for beta-TRCp-mediated ubiquitinylation and proteasomal degradation.

Protein type: CK1 group; CK1 family; EC 2.7.11.1; Kinase, protein; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: beta-catenin destruction complex; centrosome; cytosol; keratin filament; membrane; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck

Molecular Function: kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cell surface receptor linked signal transduction; Golgi organization and biogenesis; intermediate filament cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; phagocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of cell shape; Wnt receptor signaling pathway