SerpinB2 cell lysate
Human SerpinB2 Baculovirus-Insect cells Overexpression Lysate
Gene Names
SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201
Applications
Western Blot
Synonyms
SerpinB2; Human SerpinB2 Baculovirus-Insect cells Overexpression Lysate; Human SerpinB2/PAI-2 Insect Cell Lysate (WB positive control); serpin peptidase inhibitor; clade B (ovalbumin); member 2; Human HsT1201 Overexpression Lysate; Human PAI Overexpression Lysate; Human PAI-2 Overexpression Lysate; Human PAI2 Overexpression Lysate; Human PLANH2 Overexpression Lysate; Human Serpin B2 Overexpression Lysate; SerpinB2 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
640aa
Sequence
Met1-Pro415
Applicable Applications for SerpinB2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the full length of human SERPINB2 (NP_002566.1) (Met 1-Pro 415) was fused with the GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinB2 cell lysate
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in some fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
SerpinB2, also known as Plasminogen activator inhibitor 2, Placental plasminogen activator inhibitor, Monocyte Arg-serpin, Urokinase inhibitor and PAI2, is a cytoplasm protein that belongs to the serpin family and Ov-serpin subfamily. SerpinB2 is a major product of activated monocytes and macrophages and is substantially induced during most inflammatory processes. Distinct from its widely described extracellular role as an inhibitor of urokinase plasminogen activator. SerpinB2 has been shown to have an intracellular role as a retinoblastoma protein (Rb)-binding protein that inhibits Rb degradation. SerpinB2 is widely described as an inhibitor of urokinase plasminogen activator. SerpinB2 inhibits urokinase-type plasminogen activator. The monocyte derived SerpinB2 is distinct from the endothelial cell-derived PAI-1. SerpinB2 is a potentially important inducible host factor that significantly promotes HIV-1 replication.
This Human SerpinB2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB2 protein from the overexpression lysate was verified.
SerpinB2, also known as Plasminogen activator inhibitor 2, Placental plasminogen activator inhibitor, Monocyte Arg-serpin, Urokinase inhibitor and PAI2, is a cytoplasm protein that belongs to the serpin family and Ov-serpin subfamily. SerpinB2 is a major product of activated monocytes and macrophages and is substantially induced during most inflammatory processes. Distinct from its widely described extracellular role as an inhibitor of urokinase plasminogen activator. SerpinB2 has been shown to have an intracellular role as a retinoblastoma protein (Rb)-binding protein that inhibits Rb degradation. SerpinB2 is widely described as an inhibitor of urokinase plasminogen activator. SerpinB2 inhibits urokinase-type plasminogen activator. The monocyte derived SerpinB2 is distinct from the endothelial cell-derived PAI-1. SerpinB2 is a potentially important inducible host factor that significantly promotes HIV-1 replication.
This Human SerpinB2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB2 protein from the overexpression lysate was verified.
References
Sumi, Y. et al., 1989, J. Biochem. 106: 703-7. Rawlings, N.D. et al., 2004, Biochem J. 378: 705-16. Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42. Schroder, WA. et al., 2010, J Immunol. 184 (5): 2663-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,596 Da
NCBI Official Full Name
plasminogen activator inhibitor 2
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 2
NCBI Official Symbol
SERPINB2
NCBI Official Synonym Symbols
PAI; PAI2; PAI-2; PLANH2; HsT1201
NCBI Protein Information
plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin)
UniProt Protein Name
Plasminogen activator inhibitor 2
Protein Family
UniProt Gene Name
SERPINB2
UniProt Synonym Gene Names
PAI2; PLANH2; PAI-2
UniProt Entry Name
PAI2_HUMAN
Uniprot Description
SERPINB2: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Belongs to the serpin family. Ov-serpin subfamily.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: fibrinolysis; blood coagulation; negative regulation of apoptosis
Research Articles on SerpinB2
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Product Notes
The SerpinB2 serpinb2 (Catalog #AAA8114005) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 640aa. AAA Biotech's SerpinB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinB2 serpinb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro41 5. It is sometimes possible for the material contained within the vial of "SerpinB2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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