CAMKV cell lysate
Human CAMKV Baculovirus-Insect cells Overexpression Lysate
Gene Names
CAMKV; 1G5; VACAMKL
Applications
Western Blot
Synonyms
CAMKV; Human CAMKV Baculovirus-Insect cells Overexpression Lysate; Human CAMKV Insect Cell Lysate (WB positive control); CaM kinase-like vesicle-associated; Human 1G5 Overexpression Lysate; Human VACAMKL Overexpression Lysate; CAMKV cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
738aa
Sequence
Met1-Ser501
Applicable Applications for CAMKV cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CAMKV (NP_076951.2) (Met 1-Ser 501) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CAMKV cell lysate
CaM kinase-like vesicle-associated protein, also known as CAMKV, is a peripheral membrane protein and Cytoplasmic vesicle membrane protein which belongs to theprotein kinase superfamily and CAMK Ser/Thr protein kinase family. CAMKV contains oneprotein kinase domain. It is predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. CAMKV may be associated with vesicular structures. It does not appear to have detectable kinase activity. Protein kinases are a group of enzymes that move a phosphate group onto proteins, in a process called phosphorylation. Protein kinases function as an on/off switch for many cellular processes, including metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. They also function in embryonic development, physiological responses, and in the nervous and immune system. Abnormal phosphorylation causes many human diseases, including cancer, and drugs that affect phosphorylation can treat those diseases. The protein kinase domain is a structurally conserved protein domain containing the catalytic function of protein kinases. Protein kinases play a role in a mulititude of cellular processes, including division, proliferation, apoptosis, and differentiation. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins.
This Human CAMKV overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CAMKV protein from the overexpression lysate was verified.
This Human CAMKV overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CAMKV protein from the overexpression lysate was verified.
Product Categories/Family for CAMKV cell lysate
References
Hunter T, et al., 1988, Science. 241 (4861): 42-51. Wiemann S., et al., 2001, Genome Res. 11:422-435. G. Manning, et al., 2002, Science 6. 298:1912-1934. Manning G, et al., 2002, Science. 298 (5600): 1912-34. Ota T., et al., 2004, Nat. Genet. 36:40-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,354 Da
NCBI Official Full Name
caM kinase-like vesicle-associated protein
NCBI Official Synonym Full Names
CaM kinase-like vesicle-associated
NCBI Official Symbol
CAMKV
NCBI Official Synonym Symbols
1G5; VACAMKL
NCBI Protein Information
caM kinase-like vesicle-associated protein; vesicle-associated calmodulin-binding protein
UniProt Protein Name
CaM kinase-like vesicle-associated protein
Protein Family
UniProt Gene Name
CAMKV
UniProt Entry Name
CAMKV_HUMAN
Uniprot Description
VACAMKL: Does not appear to have detectable kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; CAMK-Unique family
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cytoplasmic vesicle membrane; plasma membrane
Molecular Function: calmodulin binding; ATP binding; protein kinase activity
Biological Process: protein amino acid phosphorylation
Research Articles on CAMKV
Similar Products
Product Notes
The CAMKV camkv (Catalog #AAA8114936) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 738aa. AAA Biotech's CAMKV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CAMKV camkv for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser50 1. It is sometimes possible for the material contained within the vial of "CAMKV, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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