EGFL6 active protein

EGFL6 Protein, Human, Recombinant (His & DYKDDDDK Tag)

Cat. Num. AAA8120529

• Gene Names: EGFL6; W80; MAEG
• Purity: >65% as determined by SDS-PAGE
• Synonyms: EGFL6; EGFL6 Protein; Human; Recombinant (His & DYKDDDDK Tag); Human EGFL6/EGF-L6 Protein (DYKDDDDK & His Tag); MAEG Protein; W80 Protein; EGF-like-domain; multiple 6; EGFL6 active protein

• Host: Baculovirus-insect cells
• Purity/Purification: >65% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asn22-Asp553

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to CD4-coated plates (1.25ug/mL and 100uL/well),approximately 40%-60% will adhere specifically after 30 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: N-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the human EGFL6 (NP_056322.2)(Asn22-Asp553) was expressed with the DYKDDDDK tag at the N-terminus and the histidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EGFL6 active protein

Background: EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.

References

Kimura K. et al., 2006, Genome Res. 16 (1): 55-65. Lee SH. et al., 2008, Pathology. 40 (1): 95-7. Oberauer R. et al., 2010, Mol Cell Biochem. 343 (1-2): 257-69.

NCBI and Uniprot Product Information

• NCBI GI #: 13124888
• NCBI GeneID: 25975
• NCBI Accession #: NP_056322.2
• NCBI GenBank Nucleotide #: NM_015507.3
• UniProt Accession #: Q8IUX8; Q6UXJ1; Q8NBV0; Q8WYG3; Q9NY67; Q9NZL7; Q9UFK6; B2RCB1
• Molecular Weight: 61,317 Da
• NCBI Official Full Name: epidermal growth factor-like protein 6 isoform 1
• NCBI Official Synonym Full Names: EGF-like-domain, multiple 6
• NCBI Official Symbol: EGFL6
• NCBI Official Synonym Symbols: W80; MAEG
• NCBI Protein Information: epidermal growth factor-like protein 6; EGF-like protein 6; MAM and EGF domain containing; EGF repeat-containing protein 6; MAM and EGF domains-containing gene protein
• UniProt Protein Name: Epidermal growth factor-like protein 6
• Protein Family: Epidermal growth factor-like protein
• UniProt Gene Name: EGFL6
• UniProt Synonym Gene Names: MAEG; EGF-like protein 6
• UniProt Entry Name: EGFL6_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. The gene product contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. This gene is expressed early during development, and its expression has been detected in lung and meningioma tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

EGFL6: May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; membrane; basement membrane

Molecular Function: integrin binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; multicellular organismal development; cell adhesion; cell cycle; cell differentiation