CNDP2 active protein

CNDP2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122633

• Gene Names: Cndp2; Cn2; Pep1; Dip-2; Pep-1; C76600; 0610010E05Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CNDP2; CNDP2 Protein; Mouse; Recombinant (His Tag); Mouse CNDP2/CN2/CPGL Protein (His Tag); 0610010E05Rik Protein; C76600 Protein; Cn2 Protein; Dip-2 Protein; Pep-1 Protein; Pep1 Protein; CNDP dipeptidase 2 (metallopeptidase M20 family); CNDP2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn475

• Species: Mouse
• Activity: Measured by its ability to cleave carnosine (beta-Ala-His) in a two step assay (mouse CNDP2 concentration 10ug/ml). The specific activity is >40pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CNDP2 (NP_075638.2)(Met 1-Asn 475) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNDP2 active protein

Background: Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to thepeptidase M2A family. CNDP2/CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2/CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Itmay be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform1 of CNDP2/CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform2 of CNDP2/CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2/CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.

References

Bakker,SJ. et al., 2008, Diabetes 57 (12):e16; author reply e17. Wanic, K. et al., 2008, Diabetes 57 (9): 2547-51. McDonough,CW. et al., 2009, Hum Genet 126 (2): 265-75. Kaur,H. et al., 2009, J Biol Chem. 284 (21):14493-502.

NCBI and Uniprot Product Information

• NCBI GI #: 31981273
• NCBI GeneID: 66054
• NCBI Accession #: NP_075638.2
• NCBI GenBank Nucleotide #: NM_023149.3
• UniProt Accession #: Q9D1A2; Q3TA80; Q3TI32; Q3U7B9; Q99PV1
• Molecular Weight: 52,767 Da
• NCBI Official Full Name: cytosolic non-specific dipeptidase
• NCBI Official Synonym Full Names: CNDP dipeptidase 2 (metallopeptidase M20 family)
• NCBI Official Symbol: Cndp2
• NCBI Official Synonym Symbols: Cn2; Pep1; Dip-2; Pep-1; C76600; 0610010E05Rik
• NCBI Protein Information: cytosolic non-specific dipeptidase
• UniProt Protein Name: Cytosolic non-specific dipeptidase
• Protein Family: Cytosolic non-specific dipeptidase
• UniProt Gene Name: Cndp2
• UniProt Synonym Gene Names: Cn2
• UniProt Entry Name: CNDP2_MOUSE