Nt5e active protein

Nt5e, 29-551aa, Mouse,His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206515

• Gene Names: Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Nt5e; 29-551aa; Mouse; His-tag; Baculovirus (Bioactivity Validated); 5-nucleotidase; 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5; 5-NT; Nt5e active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H
• Sequence Length: 576

• Applicable Applications for Nt5e active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Nt5e active protein

Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of general functional importance for the metabolism of nucleotides at the ventricular surface of the retina as well as the ventricles of the brain, a feature that is maintained throughout development. Recombinant mouse Nt5e, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Nt5e active protein

Cardiovascular

References

Koszalka P., et al. (2016) PLoS One. 11:e0151420.; Braun N., et al. (1995) Brain Res Dev Brain Res. 88:79-86.

NCBI and Uniprot Product Information

• NCBI GI #: 6754900
• NCBI GeneID: 23959
• NCBI Accession #: NP_035981
• NCBI GenBank Nucleotide #: NM_011851.4
• UniProt Accession #: Q61503; Q3U3S1
• Molecular Weight: 59.1kDa (531aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: 5'-nucleotidase preproprotein
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT

NCBI Description

This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]

Uniprot Description

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.