Human Forkhead Box Protein Q1 ELISA Kit | FOXQ1 elisa kit

Human Forkhead Box Protein Q1 (FOXQ1) ELISA Kit

Cat. Num. AAA2031972

• Gene Names: FOXQ1; HFH1
• Reactivity: Human
• Synonyms: Forkhead Box Protein Q1; Human Forkhead Box Protein Q1 (FOXQ1) ELISA Kit; HFH1; FOX-Q1; HNF-3/forkhead-like protein 1; Hepatocyte nuclear factor 3 forkhead homolog 1; FOXQ1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein Q1 (FOXQ1).; No significant cross-reactivity or interference between Forkhead Box Protein Q1 (FOXQ1) and analogues was observed.
• Sequence Length: 403

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein Q1 (FOXQ1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein Q1 (FOXQ1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for FOXQ1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein Q1 (FOXQ1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein Q1 (FOXQ1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein Q1 (FOXQ1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein Q1 (FOXQ1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FOXQ1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 145309320
• NCBI GeneID: 94234
• NCBI Accession #: NP_150285.3
• NCBI GenBank Nucleotide #: NM_033260.3
• Molecular Weight: 41,526 Da
• NCBI Official Full Name: forkhead box protein Q1
• NCBI Official Synonym Full Names: forkhead box Q1
• NCBI Official Symbol: FOXQ1
• NCBI Official Synonym Symbols: HFH1
• NCBI Protein Information: forkhead box protein Q1
• UniProt Protein Name: Forkhead box protein Q1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXQ1
• UniProt Synonym Gene Names: HFH1; HFH-1
• UniProt Entry Name: FOXQ1_HUMAN

NCBI Description

FOXQ1 is a member of the FOX gene family, which is characterized by a conserved 110-amino acid DNA-binding motif called the forkhead or winged helix domain. FOX genes are involved in embryonic development, cell cycle regulation, tissue-specific gene expression, cell signaling, and tumorigenesis (Bieller et al., 2001 [PubMed 11747606]).[supplied by OMIM, May 2009]

Uniprot Description

FOXQ1: Plays a role in hair follicle differentiation.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: anatomical structure morphogenesis; cell differentiation; hair follicle morphogenesis; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent