Rabbit RbBP5 Polyclonal Antibody | anti-RbBP5 antibody

RbBP5 Antibody

Cat. Num. AAA840886

• Gene Names: Rbbp5; C330016J05; 4933411J24Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: RbBP5; Polyclonal Antibody; RbBP5 Antibody; Retinoblastoma binding protein 5; RBQ3; Retinoblastoma binding protein RBQ3; anti-RbBP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 538

• Applicable Applications for anti-RbBP5 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 mug/ml). However, the optimal concentrations should be determined individually. Jurkat cell can be used as a positive control. Reacts with human/mouse/rat. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 504 of mouse RbBP5
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-RbBP5 antibody

Background: Retinoblastoma binding protein 5 (RbBP5) belongs to a highly conserved subfamily of WD-repeat proteins and it is also a subunit of a human Set1-like histone methyltransferase (HMT) complex. The encoded RbBP 5protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B

NCBI and Uniprot Product Information

• NCBI GI #: 164450487
• NCBI GeneID: 213464
• NCBI Accession #: NP_766105
• NCBI GenBank Nucleotide #: NM_172517.2
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: retinoblastoma-binding protein 5
• NCBI Official Synonym Full Names: retinoblastoma binding protein 5
• NCBI Official Symbol: Rbbp5
• NCBI Official Synonym Symbols: C330016J05; 4933411J24Rik
• NCBI Protein Information: retinoblastoma-binding protein 5
• UniProt Protein Name: Retinoblastoma-binding protein 5
• Protein Family: Retinoblastoma-binding protein
• UniProt Gene Name: Rbbp5
• UniProt Synonym Gene Names: RBBP-5
• UniProt Entry Name: RBBP5_MOUSE

Uniprot Description

RBBP5: As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with WDR5 and ASH2L; the interaction is direct. Interacts with WDR82 and SETD1A. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Cellular Component: histone methyltransferase complex; nucleolus; nucleus

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; histone H3-K4 methylation; chromatin modification; response to DNA damage stimulus