Rabbit anti-Human, Mouse Insulin-Like Growth Factor Binding Protein 4 Polyclonal Antibody | anti-IGFBP4 antibody

Insulin-Like Growth Factor Binding Protein 4, NT (IGF Binding Protein 4, IBP 4, IBP4, IBP-4, IGFBP 4, IGFBP4, IGFBP-4, BP 4, BP-4, HT29-IGFBP) (Azide free) (HRP)

Cat. Num. AAA6481889

• Gene Names: IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Insulin-Like Growth Factor Binding Protein 4; Polyclonal Antibody; NT (IGF Binding Protein 4; IBP 4; IBP4; IBP-4; IGFBP 4; IGFBP4; IGFBP-4; BP 4; BP-4; HT29-IGFBP) (Azide free) (HRP); Insulin-like Growth Factor-binding Protein 4; IGF-binding Protein 4; anti-IGFBP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IGFBP4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IGFBP4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human IGFBP4 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IGFBP4 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). IGFBP4 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(IGFBP4 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IGFBP4 antibody

Antibodies; Growth Factors; Cytokines

References

1. Durai, R., et.al., Colorectal Dis 9(7), 625-631 (2007). 2. Laursen, L.S., et.al., Mol. Endocrinol. 21(5), 1246-1257 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 62243290
• NCBI GeneID: 3487
• NCBI Accession #: NP_001543
• NCBI GenBank Nucleotide #: NM_001552
• UniProt Accession #: P22692
• Molecular Weight: 28kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 4
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 4
• NCBI Official Symbol: IGFBP4
• NCBI Official Synonym Symbols: BP-4; IBP4; IGFBP-4; HT29-IGFBP
• NCBI Protein Information: insulin-like growth factor-binding protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein 4
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP4
• UniProt Synonym Gene Names: IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
• UniProt Entry Name: IBP4_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]