Western Blot (WB)
(PUS3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUS3 antibody detected the PUS3 protein (arrow).)
Rabbit anti-Human PUS3 Polyclonal Antibody | anti-PUS3 antibody
PUS3 Antibody (N-term)
Western Blot (WB)
(PUS3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUS3 antibody detected the PUS3 protein (arrow).)
Western Blot (WB)
(PUS3 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUS3 antibody detected the PUS3 protein (arrow).)
Immunohistochemistry (IHC)
(PUS3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUS3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PUS3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUS3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Chen, J., et al. Biochemistry 39(41):12723-12730(2000)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
Uniprot Description
PUS3: Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs. Belongs to the tRNA pseudouridine synthase TruA family.
Protein type: EC 5.4.99.45; Isomerase; RNA processing; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: nucleus
Molecular Function: RNA binding; pseudouridine synthase activity
Biological Process: tRNA pseudouridine synthesis
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Product Notes
The PUS3 pus3 (Catalog #AAA9209571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 35-64. The PUS3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PUS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PUS3 pus3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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