Alpl active protein

Alpl, 19-503aa, Mouse, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206476

• Gene Names: Alpl; ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Alpl; 19-503aa; Mouse; His-tag; Baculovirus (Bioactivity Validated); Alkaline phosphatase; tissue-nonspecific isozyme; Akp-2; Akp2; ALP; APTNAP; TNAP; TNSALP; HOPS; Alpl active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH
• Sequence Length: 524

• Applicable Applications for Alpl active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Alpl active protein

Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Alpl active protein

Enzymes & Proteases; Cell Cycle

References

Whyte MP. et al., (1995) J. Clin Invest. 95:1440-1445; Brun-Heath I. et al., (2011) Cell Tissue Res. 343: 521-536.

NCBI and Uniprot Product Information

• NCBI GI #: 160333226
• NCBI GeneID: 11647
• NCBI Accession #: NP_031457
• NCBI GenBank Nucleotide #: NM_007431.3
• UniProt Accession #: P09242; Q6P1B0
• Molecular Weight: 54.5kDa (493aa) 50-70KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: alkaline phosphatase, tissue-nonspecific isozyme preproprotein
• NCBI Official Synonym Full Names: alkaline phosphatase, liver/bone/kidney
• NCBI Official Symbol: Alpl
• NCBI Official Synonym Symbols: ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP
• NCBI Protein Information: alkaline phosphatase, tissue-nonspecific isozyme
• UniProt Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme
• Protein Family: Alkaline phosphatase
• UniProt Gene Name: Alpl
• UniProt Synonym Gene Names: Akp-2; Akp2; AP-TNAP; TNSALP

NCBI Description

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

This isozyme may play a role in skeletal mineralization.