Typical Testing Data/Standard Curve (for reference only)
Rabbit Ep-CAM/CD326 ELISA Kit | Ep-CAM/CD326 elisa kit
Rabbit Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) ELISA Kit
Gene Names
Epcam; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1
Reactivity
Rabbit
Synonyms
Ep-CAM/CD326; Rabbit Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) ELISA Kit; Ep-CAM/CD326 elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes natural and recombinant Rabbit Ep-CAM/CD326. No significant cross-reactivity or interference between Rabbit Ep-CAM/CD326 and analogues was observed.
Sequence Length
315
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ep-CAM/CD326 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit Ep-CAM/CD326 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Ep-CAM/CD326. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Ep-CAM/CD326 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Ep-CAM/CD326, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Ep-CAM/CD326. You can calculate the concentration of Ep-CAM/CD326 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Ep-CAM/CD326. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Ep-CAM/CD326 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Ep-CAM/CD326, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Ep-CAM/CD326. You can calculate the concentration of Ep-CAM/CD326 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,019 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
Epcam
NCBI Official Synonym Symbols
EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1
NCBI Protein Information
epithelial cell adhesion molecule; Trop-1 protein; epithelial glycoprotein 314; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; protein 289A; tumor-associated calcium signal transducer 1
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
Epcam
UniProt Synonym Gene Names
Tacstd1; Ep-CAM; EGP314; mEGP314
UniProt Entry Name
EPCAM_MOUSE
Uniprot Description
EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: cell surface; tight junction; membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; cell junction; lateral plasma membrane
Molecular Function: protein complex binding
Biological Process: positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation; negative regulation of apoptosis
Research Articles on Ep-CAM/CD326
Similar Products
Product Notes
The Rabbit Ep-CAM/CD326 epcam (Catalog #AAA2505511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505511 ELISA Kit recognizes Rabbit Ep-CAM/CD326. It is sometimes possible for the material contained within the vial of "Ep-CAM/CD326, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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