Glutathione S Transferase, Western Blot Positive Control Protein
Glutathione S Transferase, Western Blot Positive Control (GST)
Applications
Western Blot
Purity
>97%
Synonyms
Glutathione S Transferase; Western Blot Positive Control; Western Blot Positive Control (GST); Western Blot Positive Control protein
Host
Bacterial GST (Schirosoma japonicum~27 kD) was expressed in E.coli
Purity/Purification
>97%
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer.
Applicable Applications for Glutathione S Transferase, Western Blot Positive Control protein
Suitable for Western Blot as a protein control. Other applications not tested.
Application Notes
Western Blot: Neat. Heat once and load 10ul/lane and visualize with appropriate antibodies.
Optimal dilution to be determined by the researcher.
Optimal dilution to be determined by the researcher.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Glutathione S Transferase, Western Blot Positive Control protein
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, DYKDDDDK-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Product Categories/Family for Glutathione S Transferase, Western Blot Positive Control protein
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Product Notes
The Glutathione S Transferase, Western Blot Positive Control (Catalog #AAA634354) is a Protein produced from Bacterial GST (Schirosoma japonicum~27 kD) was expressed in E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutathione S Transferase, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Suitable for Western Blot as a protein control. Other applications not tested. Western Blot: Neat. Heat once and load 10ul/lane and visualize with appropriate antibodies. Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the Glutathione S Transferase, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase, Western Blot Positive Control, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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